Murtaza Asad, Afzal Haroon, Doan Thu-Dung, Ke Guan-Ming, Cheng Li-Ting
International Program in Animal Vaccine Technology, International College, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
Centre for Immunology & Infection, Hong Kong Science and Technology Park, Hong Kong, China.
Vaccines (Basel). 2022 Oct 22;10(11):1780. doi: 10.3390/vaccines10111780.
Flagellin activates the immune system through Toll-like receptor 5 (TLR5) and can work as an adjuvant for subunit vaccines. In this study, we tested the adjuvancy of two different N-terminal fragments of flagellin, (1) FliC, residues 1-99, and (2) FliC, residues 1-176, to incorporate larger areas of the hotspot region for potentially higher levels of TLR5 activation and immune response. A truncated version of the VP2 protein (name VP2, residues 199-356) of the Infectious bursal disease virus (IBDV) was genetically linked to the flagellin constructs, and the immune response was evaluated in chickens. Results showed that both chimeric antigen-adjuvant constructs increased humoral (total IgG titers), cellular and cytokine immune response (IL-4, IFN-γ). The resulting antibody also successfully neutralized IBDV. We conclude that the N-terminus of flagellin can act as an immune activator to enhance vaccine efficacy.
鞭毛蛋白通过Toll样受体5（TLR5）激活免疫系统，并可作为亚单位疫苗的佐剂。在本研究中，我们测试了鞭毛蛋白两个不同N端片段（1）FliC（第1 - 99位氨基酸残基）和（2）FliC（第1 - 176位氨基酸残基）的佐剂活性，以纳入热点区域的更大面积，从而潜在地实现更高水平的TLR5激活和免疫反应。传染性法氏囊病病毒（IBDV）的VP2蛋白截短版本（命名为VP2，第199 - 356位氨基酸残基）与鞭毛蛋白构建体进行基因连接，并在鸡中评估免疫反应。结果表明，两种嵌合抗原 - 佐剂构建体均增强了体液免疫（总IgG滴度）、细胞免疫和细胞因子免疫反应（IL - 4、IFN - γ）。产生的抗体也成功中和了IBDV。我们得出结论，鞭毛蛋白的N端可作为免疫激活剂来增强疫苗效力。
