Su Bor Sheu, Chiu Hua Hsien, Lin Cheng Chung, Shien Jui Hung, Yin Hsien Sheng, Lee Long Huw
Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan.
Vet Immunol Immunopathol. 2011 Feb 15;139(2-4):167-75. doi: 10.1016/j.vetimm.2010.10.001. Epub 2010 Oct 28.
A recombinant fowlpox virus (rFPV/VP2) expressing infectious bursal diseases virus (IBDV) VP2 gene has been constructed. After purification and identification of rFPV/VP2, the adjuvant activity of the recombinant chicken IL-12 (rchIL-12), synthesized by our previous construct of rFPV/chIL-12, in rFPV/VP2-expressed rVP2 antigen was assessed in one-week-old specific-pathogen free chickens. The results indicated that rchIL-12 alone or rchIL-12 plus mineral oil (MO) co-administered with rVP2 antigen significantly enhanced the production of serum neutralization (SN) antibody against IBDV, compared to those with MO alone. The SN titers in groups receiving rVP2 antigen with MO alone were more inconsistent after vaccination. On the other hand, rchIL-12 significantly stimulated IFN-γ production in serum and in splenocyte cultured supernatant, suggesting that rchIL-12 alone or plus MO significantly induced a cell-mediated immune response. Finally, bursal lesion protection from very virulent IBDV (vvIBDV) challenge in chickens receiving rVP2 antigen with rchIL-12 alone or plus MO was much more effective than that with MO alone at two weeks after boosting. Taken together, rchIL-12 alone augmented in vivo the induction of a primary and also a secondary SN antibody production and a cell-mediated immunity against IBDV rVP2 antigen, which conferred the enhancement of bursal lesion protective efficacy from vvIBDV challenge. These data indicated that a potential for chIL-12 as immunoadjuvant for chicken vaccine development such as IBDV rVP2 antigen.
已构建出一种表达传染性法氏囊病病毒（IBDV）VP2基因的重组鸡痘病毒（rFPV/VP2）。对rFPV/VP2进行纯化和鉴定后，在1周龄无特定病原体鸡中评估了由我们之前构建的rFPV/chIL-12合成的重组鸡白细胞介素-12（rchIL-12）在rFPV/VP2表达的rVP2抗原中的佐剂活性。结果表明，与单独使用矿物油（MO）相比，单独使用rchIL-12或rchIL-12加矿物油（MO）与rVP2抗原共同给药可显著提高针对IBDV的血清中和（SN）抗体的产生。单独使用MO接种rVP2抗原的组中，接种后SN滴度更不一致。另一方面，rchIL-12显著刺激血清和脾细胞培养上清液中IFN-γ的产生，表明单独使用rchIL-12或加MO可显著诱导细胞介导的免疫反应。最后，在加强免疫两周后，单独使用rchIL-12或加MO接种rVP2抗原的鸡对超强毒IBDV（vvIBDV）攻击的法氏囊病变保护比单独使用MO更有效。综上所述，单独使用rchIL-12在体内增强了针对IBDV rVP2抗原的初次和二次SN抗体产生以及细胞介导免疫的诱导，这增强了对vvIBDV攻击的法氏囊病变保护效力。这些数据表明chIL-12作为鸡疫苗（如IBDV rVP2抗原）开发的免疫佐剂具有潜力。
