编码来自小鼠颌下腺的一种新型激肽释放酶的cDNA克隆序列。

Fahnestock M, Brundage S, Shooter E M

Nucleic Acids Res. 1986 Jun 25;14(12):4823-35. doi: 10.1093/nar/14.12.4823.

Mouse submaxillary gland contains many proteolytic enzymes, the most widely studied of which are the kallikreins. This gland also contains high levels of nerve growth factor (NGF), which is isolated as a complex of three subunits, alpha, beta, and gamma. We report here the cloning and sequence analysis of a novel kallikrein from mouse submaxillary gland. Antibodies directed against the alpha subunit precipitate the product of this clone, but do not precipitate the homologous gamma subunit. This new kallikrein is therefore closely related to alpha NGF, yet in contrast to the alpha subunit, its sequence suggests it has proteolytic activity.

小鼠颌下腺含有多种蛋白水解酶，其中研究最广泛的是激肽释放酶。该腺体还含有高水平的神经生长因子（NGF），它以α、β和γ三个亚基的复合物形式被分离出来。我们在此报告从小鼠颌下腺克隆并测序一种新型激肽释放酶。针对α亚基的抗体沉淀该克隆的产物，但不沉淀同源的γ亚基。因此，这种新的激肽释放酶与α-NGF密切相关，但与α亚基不同的是，其序列表明它具有蛋白水解活性。