Richards R I, Catanzaro D F, Mason A J, Morris B J, Baxter J D, Shine J
J Biol Chem. 1982 Mar 25;257(6):2758-61.
A library of cloned cDNA to male mouse submaxillary gland poly(A)-containing RNA was constructed in the plasmid pBR322. Inserts containing sequences estimated to be in the 1-5% abundance class were identified by hybridization to radiolabeled cDNA and examined by nucleotide sequence analysis. A sequence coding for a peptide with 57% homology to the only complete kallikrein sequence reported to date (from pig pancreas) was identified by a computer search program. This insert appears to code for the COOH-terminal 149 amino acids of a protein presumed therefore to be a serine protease. Comparison of the predicted amino acid sequence of this protein with analogous sequences in the three characterized members of the mouse submaxillary gland kallikrein arginyl esteropeptidase group of enzymes revealed extensive homology, although not complete identity. Thus, there are at least four members of this enzyme family expressed in the mouse submaxillary gland.
在质粒pBR322中构建了一个克隆的cDNA文库，该文库来自雄性小鼠下颌下腺含poly(A)的RNA。通过与放射性标记的cDNA杂交，鉴定出含有估计丰度在1%-5%类别的序列的插入片段，并通过核苷酸序列分析进行检查。通过计算机搜索程序鉴定出一个编码与迄今为止报道的唯一完整激肽释放酶序列（来自猪胰腺）具有57%同源性的肽的序列。该插入片段似乎编码一种蛋白质的COOH末端149个氨基酸，因此推测该蛋白质是一种丝氨酸蛋白酶。将该蛋白质的预测氨基酸序列与小鼠下颌下腺激肽释放酶精氨酰酯肽酶组的三种已鉴定成员的类似序列进行比较，发现尽管并非完全相同，但具有广泛的同源性。因此，该酶家族在小鼠下颌下腺中至少有四个成员表达。
