Chu Zhaorui, Huang Dandan, Huang Xiaoyu, He Jianfeng, Chen Lexin, Wang Jinyin, Rong Hongwei
School of Civil Engineering, Guangzhou University, Guangzhou 510006, China; Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou 510006, China.
School of Civil Engineering, Guangzhou University, Guangzhou 510006, China.
Bioresour Technol. 2023 Feb;369:128284. doi: 10.1016/j.biortech.2022.128284. Epub 2022 Nov 9.
The effective inhibition of nitrite-oxidizing bacteria (NOB) is widely acknowledged to be a critical issue for mainstream short-cut biological nitrogen removal. This study demonstrated a stable mainstream nitritation by implementing light irradiation. A sequencing batch reactor with ultraviolet-A (UVA) irradiation was operated for 250 days, and a high nitrite accumulation ratio was achieved and stabilized at about 90 %. UVA irradiation also positively impacts denitrification activity, with total nitrogen removal up to 63 %. Microbial community analysis confirmed that the UVA effectively and stably decreased the abundance of Nitrospira (the only detected NOB) from 6.0 % to 0.1 %, while it showed no effect on Nitrosomonas. The enriched genus Rhodocyclaceae was the major contributor to the increase in denitrification activity in the light-induced nitritation system. The proposed UVA irradiation strategy has the potential to be integrated with an anoxic/aerobic (A/O) or integrated fixed-film activated sludge (IFAS) process for achieving mainstream short-cut biological nitrogen removal.
有效抑制亚硝酸盐氧化细菌（NOB）被广泛认为是主流短程生物脱氮的关键问题。本研究通过实施光照证明了稳定的主流亚硝化作用。运行一个配备紫外线A（UVA）照射的序批式反应器250天，实现了高亚硝酸盐积累率并稳定在约90%。UVA照射对反硝化活性也有积极影响，总氮去除率高达63%。微生物群落分析证实，UVA有效且稳定地将硝化螺菌属（唯一检测到的NOB）的丰度从6.0%降至0.1%，而对亚硝化单胞菌没有影响。富集的红环菌科是光诱导亚硝化系统中反硝化活性增加的主要贡献者。所提出的UVA照射策略有可能与缺氧/好氧（A/O）或一体式固定膜活性污泥（IFAS）工艺相结合，以实现主流短程生物脱氮。
