Salauddin Asma, Chakma Kallyan, Hasan Md Mahbub, Akter Farhana, Chowdhury Nowshad Asgar, Chowdhury Sumon Rahman, Mannan Adnan
Department of Genetic Engineering & Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chattogram, 4331, Bangladesh.
Disease Biology and Molecular Epidemiology Research Group, Chattogram, Bangladesh.
Mol Biol Rep. 2023 Jan;50(1):609-619. doi: 10.1007/s11033-022-08081-x. Epub 2022 Nov 11.
Diabetes is a severe health burden for Bangladesh. Genetic polymorphism has been reported to be one of the major risk factors for diabetes in various studies. TCF7L2 (transcription factor 7 like 2) transcripts in the human β-cell have effects on β-cell survival, function, and Wnt signaling activation. This study aimed to evaluate the frequency and association of various polymorphisms namely TCF7L2 rs12255372 and rs7903146 among Bangladeshi patients with T2DM (Type 2 Diabetes Mellitus).
This case-control study included 300 patients with T2DM and 234 healthy individuals from two health facilities in the Chattogram Division of Bangladesh. Anthropometric measurements were assessed using a self-reported, structured, eight-item questionnaire. The polymorphisms were identified by PCR-RFLP and sequencing method.
A strong association of T2DM with polymorphisms was observed, including rs12255372 (p = 0.0004) and rs7903146 (p = 0.005). It was observed that the risk genotype at rs12255372 was associated with age (p = 0.009), a family history of diabetes (p < 0.0001), and HbA1C (p < 0.0001). Furthermore, it was found that rs12255372 was substantially associated with hypertension (p = 0.03), eye problems (p = 0.01), and neurological abnormalities (p = 0.02).
This study postulates that TCF7L2 genetic polymorphism is associated with the risk of T2DM among the studied Bangladeshi population. The findings should be replicated through more studies with a large number of samples and in different populations.
糖尿病是孟加拉国沉重的健康负担。在各项研究中，基因多态性已被报道为糖尿病的主要风险因素之一。人β细胞中的TCF7L2（转录因子7样2）转录本对β细胞存活、功能及Wnt信号激活有影响。本研究旨在评估孟加拉国2型糖尿病（T2DM）患者中各种多态性即TCF7L2 rs12255372和rs7903146的频率及关联性。
这项病例对照研究纳入了来自孟加拉国吉大港区两个医疗机构的300例T2DM患者和234名健康个体。使用一份自我报告的、结构化的八项问卷评估人体测量指标。通过PCR-RFLP和测序方法鉴定多态性。
观察到T2DM与多态性之间存在强关联，包括rs12255372（p = 0.0004）和rs7903146（p = 0.005）。观察到rs12255372处的风险基因型与年龄（p = 0.009）、糖尿病家族史（p < 0.0001）及糖化血红蛋白（p < 0.0001）相关。此外，发现rs12255372与高血压（p = 0.03）、眼部问题（p = 0.01）及神经异常（p = 0.02）显著相关。
本研究推测，在被研究的孟加拉国人群中，TCF7L2基因多态性与T2DM风险相关。这些发现应通过更多大样本研究及在不同人群中进行重复验证。
