Dobbs L G, Gonzalez R, Williams M C
Am Rev Respir Dis. 1986 Jul;134(1):141-5. doi: 10.1164/arrd.1986.134.1.141.
A method has been developed for isolating alveolar type II cells by digesting lung tissue with elastase and "panning" the resultant cell suspension on plates coated with IgG. This method provides both high yield and purity of type II cells. In 50 experiments with rats, we obtained 35 +/- 11 X 10(6) cells/rat, 89 +/- 4% of which were type II cells (mean +/- SD). Type II cells isolated by "panning" adhered more rapidly and completely in tissue culture than did cells isolated by centrifugation over discontinuous density gradients of metrizamide. The "panning" method is superior to other methods for isolating type II cells in that it provides a population of type II cells of both high yield and high purity. The method is fast, reproducible, and easily adaptable to isolating type II cells from species other than rats.
已开发出一种通过用弹性蛋白酶消化肺组织并将所得细胞悬液铺在包被有IgG的平板上“淘选”来分离肺泡II型细胞的方法。该方法可提供高产率和高纯度的II型细胞。在对大鼠进行的50次实验中，我们每只大鼠获得35±11×10⁶个细胞，其中89±4%为II型细胞（平均值±标准差）。通过“淘选”分离的II型细胞在组织培养中比通过在不连续的甲泛葡胺密度梯度上离心分离的细胞更快且更完全地贴壁。“淘选”方法优于其他分离II型细胞的方法，因为它能提供高产率和高纯度的II型细胞群体。该方法快速、可重复，并且易于适用于从大鼠以外的物种分离II型细胞。
