Clinical Laboratories Sciences Department, the College of Applied Medical Sciences, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia; Division Evolution, Infection and Genomic Sciences, The University of Manchester, Manchester M13 9PT, UK.
Division Evolution, Infection and Genomic Sciences, The University of Manchester, Manchester M13 9PT, UK.
J Virol Methods. 2023 Feb;312:114651. doi: 10.1016/j.jviromet.2022.114651. Epub 2022 Nov 9.
Modified vaccinia virus Ankara (MVA) is an attenuated vaccinia virus with restricted replication in human cells. The virus serves as an ideal vaccine vector suitable for safe use even in immune-compromised individuals. With its inherently large packaging capacity, expression cassettes encoding bulky genes can be inserted into deletion regions within the MVA genome. These deletion sites develop during the process of the attenuation of the virus by passage in Chicken Embryo Fibroblasts (pCEFs). Transgene stability in MVA is important to assure immunogenicity and efficacy. In the present study, we assessed the effect of substantial passage of recombinant MVA vectors on the stability of expression cassette encoding SIV Gag/Tat genes inserted at the Del-II site, as part of generating a vaccine to protect from HIV. Our data indicated that after 15 passages there was a significant loss or mutation of the inserted genes.
改良安卡拉痘苗病毒（MVA）是一种减毒的痘苗病毒，在人类细胞中的复制受到限制。该病毒作为一种理想的疫苗载体，即使在免疫功能低下的个体中也能安全使用。由于其固有的大容量包装能力，可以将编码大基因的表达盒插入 MVA 基因组的缺失区域。这些缺失位点是在鸡胚成纤维细胞（pCEFs）中通过病毒传代衰减过程中产生的。MVA 中转基因的稳定性对于确保免疫原性和疗效非常重要。在本研究中，我们评估了大量传代重组 MVA 载体对插入 Del-II 位点的 SIV Gag/Tat 基因表达盒稳定性的影响，这是生成一种预防 HIV 的疫苗的一部分。我们的数据表明，在 15 次传代后，插入的基因显著丢失或发生突变。
