Northwest A&F University Shenzhen Research Institute, Shenzhen, Guangdong, 518057, China; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Northwest A&F University Shenzhen Research Institute, Shenzhen, Guangdong, 518057, China; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Fish Shellfish Immunol. 2022 Dec;131:1101-1108. doi: 10.1016/j.fsi.2022.11.011. Epub 2022 Nov 11.
DNA vaccines, as an effective prophylactic technology to induce both humoral and cellular immune responses, have already been widely studied to prevent and control viral and bacterial infections in aquaculture. To find a more effective and safer way to control Micropterus salmoides rhabdovirus (MSRV) infection in largemouth bass, two different DNA vaccines expressing partial (pcDNA3.1-G2) and full-length (pcDNA3.1-G) of the MSRV G protein were developed and injected intramuscularly with different doses. The immune effect was comprehensively compared and evaluated by detecting immune-related parameters including serum antibody levels, immune-related physiological indexes, immune-related gene expression and relative survival rates in this study. The results showed that compared with the pcDNA3.1-G vaccine, the pcDNA3.1-G2 vaccine induced higher serum antibody levels, a lower nonspecific immune response in serum (ACP, SOD and T-AOC activities), higher immune-related gene expression and a higher relative survival rate. Moreover, the immune effect of pcDNA3.1-G2-vaccinated fish showed gradually higher with the increasing pcDNA3.1-G2 concentration, especially in pcDNA3.1-G2 (10μg/per fish) group, the relative survival rate reached to 82.5%, which was significant higher (p < 0.05) than pcDNA3.1-G (10μg/per fish) group. This study indicated that screening the potential core part of an antigen is an achievable strategy to improve the immunogenicity and immunoprotective effect of DNA vaccine.
DNA 疫苗作为一种有效的预防性技术，可以诱导体液和细胞免疫反应，已广泛研究用于预防和控制水产养殖中的病毒和细菌感染。为了寻找更有效和更安全的方法来控制大口黑鲈弹状病毒（MSRV）感染，本研究构建了两种不同的表达 MSRV G 蛋白部分（pcDNA3.1-G2）和全长（pcDNA3.1-G）的 DNA 疫苗，通过肌肉注射不同剂量的疫苗来进行免疫。通过检测血清抗体水平、免疫相关生理指标、免疫相关基因表达和相对存活率等免疫相关参数，综合比较和评估了两种疫苗的免疫效果。结果表明，与 pcDNA3.1-G 疫苗相比，pcDNA3.1-G2 疫苗诱导了更高的血清抗体水平，更低的血清非特异性免疫反应（ACP、SOD 和 T-AOC 活性），更高的免疫相关基因表达和更高的相对存活率。此外，pcDNA3.1-G2 疫苗的免疫效果随着 pcDNA3.1-G2 浓度的增加而逐渐提高，尤其是在 pcDNA3.1-G2（10μg/尾）组中，相对存活率达到 82.5%，显著高于 pcDNA3.1-G（10μg/尾）组（p<0.05）。本研究表明，筛选抗原的潜在核心部分是提高 DNA 疫苗免疫原性和免疫保护效果的可行策略。
