通过用非免疫兔血清进行荧光染色来观察中心粒和基体。
Visualization of centrioles and basal bodies by fluorescent staining with nonimmune rabbit sera.
作者信息
Connolly J A, Kalnins V I
出版信息
J Cell Biol. 1978 Nov;79(2 Pt 1):526-32. doi: 10.1083/jcb.79.2.526.
Abstract
Several sera from nonimmunized rabbits have been found which stain centrioles and basal bodies by indirect immunofluorescence in a wide variety of cell types. So far, approximately 10% of the rabbit sera that we have examined gave strong positive staining of centrioles and basal bodies. Cytoplasmic networks, mitotic spindles, and ciliary axonemes, however, remain unstained. This specific fluorescent staining of centrioles and basal bodies could not be abolished by absorption of sera with purified brain tubulin. This technique is superior to previous methods for the visualization of basal bodies and centrioles at the light microscopic level and should be useful for rapid and convenient detection of these organelles in large populations of cells.
摘要
已发现几例来自未免疫兔子的血清，通过间接免疫荧光法可在多种细胞类型中对中心粒和基体进行染色。到目前为止，我们检测的兔子血清中约有10%对中心粒和基体呈现强阳性染色。然而，细胞质网络、有丝分裂纺锤体和纤毛轴丝仍未被染色。用纯化的脑微管蛋白吸收血清并不能消除中心粒和基体的这种特异性荧光染色。该技术在光学显微镜水平上优于先前用于观察基体和中心粒的方法，并且对于在大量细胞中快速方便地检测这些细胞器应该是有用的。
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