中心粒和基体上抗原相关多肽的鉴定。

Identification of antigenically related polypeptides at centrioles and basal bodies.

作者信息

Lin W, Fung B, Shyamala M, Kasamatsu H

出版信息

Proc Natl Acad Sci U S A. 1981 Apr;78(4):2373-7. doi: 10.1073/pnas.78.4.2373.

DOI:10.1073/pnas.78.4.2373

PMID:6166008

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC319348/

Abstract

An antigen localized at the centriolar region has been identified by indirect immunofluorescence studies in African green monkey kidney, human, hamster, rat, and mouse cells. The antigen consists of two polypeptides of 14,000 and 17,000 daltons. A related antigen is also present at the basal body region in ciliated cells from chicken, cat, mouse, pig, steer, and rabbit trachea and from rabbit fimbria. Immunoelectron microscopy shows that the immunoreactive antigen is indeed located in the region around the basal bodies of ciliated cat tracheal cells. Thus, we have found an antigen that is common to a variety of cell types from many different animal sources and is specifically associated with both centrioles and basal bodies. The possible role of the antigen in differentiation is discussed.

摘要

通过间接免疫荧光研究，在非洲绿猴肾细胞、人细胞、仓鼠细胞、大鼠细胞和小鼠细胞中鉴定出一种定位于中心粒区域的抗原。该抗原由两条分子量分别为14,000和17,000道尔顿的多肽组成。在来自鸡、猫、小鼠、猪、牛和兔气管的纤毛细胞以及兔菌毛中，相关抗原也存在于基体区域。免疫电子显微镜显示，免疫反应性抗原确实位于猫气管纤毛细胞基体周围区域。因此，我们发现了一种抗原，它存在于许多不同动物来源的多种细胞类型中，并且与中心粒和基体都有特异性关联。文中讨论了该抗原在分化过程中可能发挥的作用。