Computational Biotechnology, RWTH-Aachen University, Worringerweg 3, 52072, Aachen, Germany.
Chem Asian J. 2023 Jan 3;18(1):e202200916. doi: 10.1002/asia.202200916. Epub 2022 Dec 13.
In this review, functionalized nanogaps embedded in nanopores are discussed in view of their high biosensitivity in detecting biomolecules, their length, type, and sequence. Specific focus is given on nanoelectrodes functionalized with tiny nanometer-sized diamond-like particles offering vast functionalization possibilities for gold junction electrodes. This choice of the functionalization, in turn, offers nucleotide-specific binding possibilities improving the detection signals arising from such functionalized electrodes potentially embedded in a nanopore. The review sheds light onto the use and enhancement of the tunnelling recognition in functionalized nanogaps towards sensing DNA nucleotides and mutation detection, providing important input for a practical realization.
在这篇综述中，讨论了嵌入在纳米孔中的功能化纳米间隙，因为它们在检测生物分子方面具有很高的生物灵敏度，以及它们的长度、类型和序列。特别关注的是用微小的纳米级类金刚石颗粒功能化的纳米电极，为金结电极提供了广泛的功能化可能性。这种功能化的选择反过来又提供了核苷酸特异性结合的可能性，从而提高了潜在嵌入在纳米孔中的这种功能化电极产生的检测信号。该综述阐明了在功能化纳米间隙中使用和增强隧穿识别以感应 DNA 核苷酸和突变检测的方法，为实际实现提供了重要的依据。
