高效液相色谱法中章鱼胺的荧光检测及其在人血清多巴胺β-单加氧酶测定中的应用

Fluorimetric detection of octopamine in high-performance liquid chromatography and its application to the assay of dopamine beta-monooxygenase in human serum.

作者信息

Flatmark T, Skotland T, Ljones T, Ingebretsen O C

出版信息

J Chromatogr. 1978 Nov 1;146(3):433-8. doi: 10.1016/s0378-4347(00)81201-3.

Abstract

A high-performance liquid chromatographic procedure is described for the determination of octopamine. The method, which is based on the separation on a microparticulate bonded strong cation-exchange resin and measurement of the native fluorescence, has been applied to give a sensitive assay of dopamine beta-monooxygenase (EC 1.14.17.1) activity in human serum with tyramine as the substrate. The procedure, which has been designed for use with an automatic sampler, has a detection limit of about 50 pmoles of octopamine, and the analysis time is approximately 10 min per sample.

摘要

描述了一种用于测定章鱼胺的高效液相色谱方法。该方法基于在微粒键合强阳离子交换树脂上的分离以及对天然荧光的测量，已被应用于以酪胺为底物对人血清中多巴胺β-单加氧酶（EC 1.14.17.1）活性进行灵敏测定。该方法设计用于与自动进样器配合使用，章鱼胺的检测限约为50皮摩尔，每个样品的分析时间约为10分钟。