Markianos E S, Nyström I E
Z Klin Chem Klin Biochem. 1975 Jul;13(7):273-6. doi: 10.1515/cclm.1975.13.7.273.
A method for the estimation of dopamine beta-hydroxylase activity in human serum is described, based on a thin layer chromatographic separation of the substrate ([14C]tyramine) from the reaction product ([14C]octopamine). The basic properties of the human serum enzyme, investigated by this method are described.
本文描述了一种基于薄层色谱法从反应产物([14C]去甲肾上腺素)中分离底物([14C]酪胺)来估算人血清中多巴胺β-羟化酶活性的方法。文中还描述了用该方法研究的人血清酶的基本特性。