Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China.
Laboratory Animals Center, Nantong University, Nantong, China.
FASEB J. 2022 Dec;36(12):e22656. doi: 10.1096/fj.202200943R.
Traumatic spinal cord injury (tSCI) is a severe injury of the central nervous system (CNS) with complicated pathological microenvironment that results in hemorrhage, inflammation, and scar formation. The microenvironment of the injured spinal cord comprises heterogeneous neurons, glial cells, inflammatory cells, and stroma-related cells. Increasing evidence has indicated that the altered cellular and molecular microenvironment following tSCI is a key factor impeding functional recovery. Single-cell RNA sequencing (scRNA-seq) has provided deep insights into the dynamic cellular and molecular changes in the microenvironment by comprehensively characterizing the diversity of spinal cord cell types. Specifically, scRNA-seq enables the exploration of the molecular mechanisms underlying tSCI by elucidating intercellular communication in spinal cord samples between normal and injury conditions at a single-cell resolution. Here, we first described the pathological and physiological processes after tSCI and gave a brief introduction of the scRNA-seq technology. We then focused on the recent scRNA-seq researches in tSCI, which characterized diverse cell-type populations and specific cell-cell interactions in tSCI. In addition, we also highlighted some potential directions for the research of scRNA-seq in tSCI in the future.
创伤性脊髓损伤（tSCI）是一种严重的中枢神经系统（CNS）损伤，其复杂的病理微环境导致出血、炎症和瘢痕形成。损伤脊髓的微环境包含异质神经元、神经胶质细胞、炎性细胞和基质相关细胞。越来越多的证据表明，tSCI 后改变的细胞和分子微环境是阻碍功能恢复的关键因素。单细胞 RNA 测序（scRNA-seq）通过全面描述脊髓细胞类型的多样性，深入了解微环境中细胞和分子的动态变化。具体来说，scRNA-seq 通过在单细胞分辨率下阐明正常和损伤条件下脊髓样本中细胞间的通讯，揭示 tSCI 背后的分子机制。在这里，我们首先描述了 tSCI 后的病理和生理过程，并简要介绍了 scRNA-seq 技术。然后，我们重点介绍了 tSCI 中最近的 scRNA-seq 研究，这些研究描述了 tSCI 中不同的细胞群体和特定的细胞间相互作用。此外，我们还强调了 scRNA-seq 在 tSCI 未来研究中的一些潜在方向。
