离体灌注系统分析趋化因子驱动的白细胞黏附。

Ex Vivo Perfusion System to Analyze Chemokine-Driven Leukocyte Adhesion.

机构信息

Interdisciplinary Centre for Clinical Research (IZKF), RWTH Aachen University, Aachen, Germany.

Institute for Molecular Cardiovascular Research (IMCAR), RWTH Aachen University, Aachen, Germany.

出版信息

Methods Mol Biol. 2023;2597:59-75. doi: 10.1007/978-1-0716-2835-5_6.

DOI:10.1007/978-1-0716-2835-5_6

PMID:36374414

Abstract

At the moment, many researchers are using in vitro techniques to investigate chemokine-driven leukocyte adhesion/recruitment, for example, by using a transwell or flow chamber system. Here we describe a more physiologically relevant, sophisticated, and highly flexible method to study leukocyte adhesion ex vivo in fresh murine carotid arteries under arterial flow conditions. This model mimics an in vivo situation and allows the combination of leukocytes and arteries isolated from different donors in one experiment, generating information on both vascular and leukocyte adhesive properties of both donors. This method provides a versatile, highly physiologically relevant model to investigate leukocyte adhesion.

摘要

目前，许多研究人员正在使用体外技术来研究趋化因子驱动的白细胞黏附/募集，例如使用 Transwell 或流动腔系统。在这里，我们描述了一种更符合生理、更复杂、更灵活的方法，用于在动脉血流条件下在新鲜的鼠颈动脉中离体研究白细胞黏附。该模型模拟了体内情况，并允许在一个实验中将来自不同供体的白细胞和动脉组合在一起，从而提供关于两个供体的血管和白细胞黏附特性的信息。该方法提供了一种多功能、高度生理相关的模型，用于研究白细胞黏附。