van der Vorst Emiel P C, Maas Sanne L, Ortega-Gomez Almudena, Hameleers Jeroen M M, Bianchini Mariaelvy, Asare Yaw, Soehnlein Oliver, Döring Yvonne, Weber Christian, Megens Remco T A
Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany.
Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands.
Bio Protoc. 2017 Jun 20;7(12). doi: 10.21769/BioProtoc.2344.
The main purpose of this sophisticated and highly versatile method is to visualize and quantify structural vessel wall properties, cellular recruitment, and lipid/dextran extravasation under physiological conditions in living arteries. This will be of interest for a broad range of researchers within the field of inflammation, hypertension, atherosclerosis, and even the pharmaceutical industry. Currently, many researchers are using techniques to evaluate cellular recruitment, like transwell or flow chamber systems with cultured cells, with unclear physiological comparability. The here introduced method describes in detail the use of a sophisticated and flexible method to study arterial wall properties and leukocyte recruitment in fresh and viable murine carotid arteries under arterial flow conditions. This model mimics the situation and allows the use of cells and arteries isolated from two different donors (for example, wildtype vs. specific knockouts) to be combined into one experiments, thereby providing information on both leukocyte and/or endothelial cell properties of both donors. As such, this model can be considered an alternative for the complicated and invasive studies, such as parabiotic experiments.
这种精密且用途广泛的方法的主要目的是在生理条件下，对活体动脉的血管壁结构特性、细胞募集以及脂质/葡聚糖外渗进行可视化和定量分析。这将引起炎症、高血压、动脉粥样硬化领域乃至制药行业的众多研究人员的兴趣。目前，许多研究人员正在使用诸如transwell或带有培养细胞的流动腔系统等技术来评估细胞募集，但生理可比性尚不清楚。本文介绍的方法详细描述了一种精密且灵活的方法，用于在动脉血流条件下研究新鲜且有活力的小鼠颈动脉的动脉壁特性和白细胞募集。该模型模拟了实际情况，并允许将从两个不同供体（例如，野生型与特定基因敲除型）分离的细胞和动脉组合到一个实验中，从而提供有关两个供体的白细胞和/或内皮细胞特性的信息。因此，该模型可被视为复杂且有创研究（如联体实验）的替代方法。
