Bloksgaard Maria, Leurgans Thomas M, Nissen Inger, Jensen Pia Søndergaard, Hansen Maria Lyck, Brewer Jonathan R, Bagatolli Luis A, Marcussen Niels, Irmukhamedov Akhmadjon, Rasmussen Lars Melholt, De Mey Jo G R
Department of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
J Vasc Res. 2015;52(1):1-11. doi: 10.1159/000376548. Epub 2015 Mar 31.
Peripheral vascular resistance is increased in essential hypertension. This involves structural changes of resistance arteries and stiffening of the arterial wall, including remodeling of the extracellular matrix. We hypothesized that biopsies of the human parietal pericardium, obtained during coronary artery bypass grafting or cardiac valve replacement surgeries, can serve as a source of resistance arteries for structural research in cardiovascular disease patients. We applied two-photon excitation fluorescence microscopy to study the parietal pericardium and isolated pericardial resistance arteries with a focus on the collagen and elastin components of the extracellular matrix. Initial findings in pig tissue were confirmed in patient biopsies. The microarchitecture of the internal elastic lamina in both the pig and patient pericardial resistance arteries (studied at a transmural pressure of 100 mm Hg) is fiber like, and no prominent external elastic lamina could be observed. This microarchitecture is very different from that in rat mesenteric arteries frequently used for resistance artery research. In conclusion, we add three-dimensional information on the structure of the extracellular matrix in resistance arteries from cardiovascular disease patients and propose further use of patient pericardial resistance arteries for studies of the human microvasculature.
原发性高血压患者的外周血管阻力会增加。这涉及阻力动脉的结构变化以及动脉壁的硬化，包括细胞外基质的重塑。我们推测，在冠状动脉搭桥手术或心脏瓣膜置换手术期间获取的人壁层心包活检组织，可作为心血管疾病患者进行结构研究的阻力动脉来源。我们应用双光子激发荧光显微镜来研究壁层心包和分离的心包阻力动脉，重点关注细胞外基质的胶原蛋白和弹性蛋白成分。在猪组织中的初步发现已在患者活检中得到证实。猪和患者心包阻力动脉（在100 mmHg的跨壁压力下研究）的内弹性膜微观结构呈纤维状，未观察到明显的外弹性膜。这种微观结构与常用于阻力动脉研究的大鼠肠系膜动脉的微观结构非常不同。总之，我们补充了心血管疾病患者阻力动脉细胞外基质结构的三维信息，并提议进一步利用患者心包阻力动脉来研究人类微血管系统。
