BioNanoTec LTD, Nicosia, Cyprus; Metropolitan College, Faculty of Engineering and Architecture, Athens, Greece.
School of Sciences, European University Cyprus, Cyprus; E.U.C. Research Centre LTD, Nicosia, Cyprus.
Micron. 2023 Jan;164:103384. doi: 10.1016/j.micron.2022.103384. Epub 2022 Nov 4.
When testing soft biological samples using the Atomic Force Microscopy (AFM) nanoindentation method, the force-indentation data is usually fitted to the equations provided by Hertzian mechanics. Nevertheless, a significant question remains up to date; is this a correct approach from a mathematical perspective? Biological materials are heterogeneous, so 'what do we calculate' when using a classic fitting approach? In this paper, conclusive answers to the abovementioned questions are provided. In addition, a new tool for the nanomechanical characterization of biological samples, the depth-dependent mechanical properties maps, is introduced.
当使用原子力显微镜（AFM）纳米压痕法测试软生物样本时，通常将力-压痕数据拟合到赫兹力学提供的方程中。然而，从数学角度来看，这是否是一种正确的方法，至今仍是一个悬而未决的问题。生物材料是不均匀的，因此在使用经典拟合方法时，“我们在计算什么”？本文对上述问题给出了明确的答案。此外，还引入了一种新的生物样本纳米力学特性分析工具，即深度相关力学特性图谱。
