School of Life Sciences, Qinghai Normal University, Xining, 810008, China; Academy of Plateau Science and Sustainability, Qinghai Normal University, Xining, 810008, China; Key Laboratory of Tibetan Plateau Medicinal Plant and Animal Resources, Qinghai Normal University, Xining, 810008, China.
School of Life Sciences, Qinghai Normal University, Xining, 810008, China.
J Plant Physiol. 2022 Dec;279:153856. doi: 10.1016/j.jplph.2022.153856. Epub 2022 Oct 31.
Lycium chinense is an important medicinal plant in the northwest of China. Flavonoids are the major pharmacological components of L. chinense fruits. However, flavonoid metabolism during fruit development of L. chinense remains to be studied. Here, we analyzed the change of flavonoid contents, enzyme activity, and gene expression during fruit development of L. chinense. We found that flavonoids, anthocyanins, and catechins are the most important components of L. chinense fruits. Flavonoid content was increased with fruit development and was high at the late developmental stage. PAL, CHS, and F3H enzymes played a significant role in flavonoid accumulation in fruits. Transcriptomic analysis showed that anthocyanin pathway, flavonol pathway, flavonoid biosynthesis, and phenylpropanoid synthesis pathway were the major pathways involved in flavonoid metabolism in L. chinense. Gene expression analysis indicated that PAL1 and CHS2 genes were critical for flavonoid metabolism in L. chinense fruits. These discoveries help us understand the dynamic changes in flavonoids during fruit development and enhance the use of L. chinense fruits.
宁夏枸杞是中国西北地区的一种重要药用植物。类黄酮是宁夏枸杞果实的主要药理成分。然而，宁夏枸杞果实发育过程中的类黄酮代谢仍有待研究。在这里，我们分析了宁夏枸杞果实发育过程中类黄酮含量、酶活性和基因表达的变化。我们发现，类黄酮、花青素和儿茶素是宁夏枸杞果实中最重要的成分。类黄酮含量随果实发育而增加，在发育后期达到高峰。PAL、CHS 和 F3H 酶在果实中类黄酮的积累中起着重要作用。转录组分析表明，花青素途径、类黄酮途径、类黄酮生物合成和苯丙烷合成途径是宁夏枸杞中类黄酮代谢的主要途径。基因表达分析表明，PAL1 和 CHS2 基因对宁夏枸杞果实中的类黄酮代谢至关重要。这些发现有助于我们了解果实发育过程中类黄酮的动态变化，并提高宁夏枸杞果实的利用价值。
