Laboratório de Citogenética, Centro de Estudos Avançados da Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal do Pará (UFPA), Belém, Pará, Brazil.
Departamento de Morfofuncional, Universidade do Estado do Pará, Tucuruí, Pará, Brazil.
Sci Rep. 2022 Nov 14;12(1):19514. doi: 10.1038/s41598-022-21391-1.
The subfamily Phyllostominae (Chiroptera, Phyllostomidae) comprises 10 genera of Microchiroptera bats from the Neotropics. The taxonomy of this group is controversial due to incongruities in the phylogenetic relationships evident from different datasets. The genus Lophostoma currently includes eight species whose phylogenetic relationships have not been resolved. Integrative analyzes including morphological, molecular and chromosomal data are powerful tools to investigate the phylogenetics of organisms, particularly if obtained by chromosomal painting. In the present work we performed comparative genomic mapping of three species of Lophostoma (L. brasiliense 2n = 30, L. carrikeri 2n = 26 and L. schulzi 2n = 26), by chromosome painting using whole chromosome probes from Phyllostomus hastatus and Carollia brevicauda; this included mapping interstitial telomeric sites. The karyotype of L. schulzi (LSC) is a new cytotype. The species L. brasiliense and L. carrikeri showed interstitial telomeric sequences that probably resulted from expansions of repetitive sequences near pericentromeric regions. The addition of chromosomal painting data from other species of Phyllostominae allowed phylogeny construction by maximum parsimony, and the determination that the genera of this subfamily are monophyletic, and that the genus Lophostoma is paraphyletic. Additionally, a review of the taxonomic status of LSC is suggested to determine if this species should be reclassified as part of the genus Tonatia.
鳞甲目（翼手目，鳞甲蝠科）的 Phyllostominae 亚科包含 10 个新大陆小型蝙蝠属。由于不同数据集显示的系统发育关系不一致，该类群的分类学存在争议。Lophostoma 属目前包括 8 个物种，其系统发育关系尚未解决。综合分析包括形态学、分子和染色体数据，是研究生物系统发育的有力工具，特别是如果通过染色体着色获得。在本工作中，我们使用来自 Phyllostomus hastatus 和 Carollia brevicauda 的整条染色体探针，通过染色体着色，对 3 种 Lophostoma（L. brasiliense 2n=30、L. carrikeri 2n=26 和 L. schulzi 2n=26）进行了比较基因组作图，包括着丝粒间端粒位点的作图。L. schulzi（LSC）的核型是一种新的细胞型。L. brasiliense 和 L. carrikeri 物种显示出着丝粒间端粒序列，可能是由于近着丝粒区重复序列的扩张所致。添加来自其他 Phyllostominae 物种的染色体着色数据，允许通过最大简约法构建系统发育，确定该亚科的属是单系的，而 Lophostoma 属是并系的。此外，建议对 LSC 的分类地位进行审查，以确定该物种是否应重新归类为 Tonatia 属的一部分。
