School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
Department of Food Science and Biotechnology, Brawijaya University, Malang 65145, Indonesia.
J Microbiol Biotechnol. 2022 Dec 28;32(12):1589-1598. doi: 10.4014/jmb.2210.10009. Epub 2022 Oct 21.
Microfibrillated cellulose (MFC) is a valuable material with wide industrial applications, particularly for the food and cosmetics industries, owing to its excellent physiochemical properties. Here, we prepared high-solid microfibrillated cellulose (HMFC) from the centrifugation of -derived MFC right after fibrillation. Dispersion properties, morphology, and structural changes were monitored during processing. HMFC has a five-fold higher solid concentration than MFC without significant changes to dispersion properties. SEM images and FTIR spectra of HMFC revealed a stable surface and structure against centrifugal forces. HMFC exhibited 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, although it could not scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH). Moreover, HMFC inhibited the generation of LPS-induced excessive nitrite and radial oxygen species in murine macrophage RAW264.7 cells. Additionally, HMFC suppressed LPS-induced Keap-1 expression in the cytosol but did not alter iNOS expression. HMFC also attenuated the UVB-induced phosphorylation of p38, c-Jun N-terminal kinase (JNK) 1/2, and extracellular-signal-regulated kinase (ERK) 1/2, as well as the phosphorylation of c-Jun in the immortalized human skin keratinocyte HaCaT cells. Therefore, the application of centrifugation is suitable for producing high-solid MFC as a candidate material for anti-inflammatory and anti-oxidative marine cosmeceuticals.
微原纤纤维素（MFC）是一种具有广泛工业应用价值的材料，特别是在食品和化妆品行业，因为其具有优异的物理化学性质。在这里，我们在纤维细化后立即通过离心从 MFC 中制备了高浓度微原纤纤维素（HMFC）。在加工过程中监测了分散性能、形态和结构变化。与 MFC 相比，HMFC 的固体浓度高五倍，但分散性能没有明显变化。HMFC 的 SEM 图像和 FTIR 光谱显示，其表面和结构对离心力具有稳定性。HMFC 具有 2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除活性，尽管它不能清除 2,2-二苯基-1-苦基肼（DPPH）。此外，HMFC 抑制了 LPS 诱导的小鼠巨噬细胞 RAW264.7 细胞中过量亚硝酸盐和径向氧物种的产生。此外，HMFC 抑制了细胞质中 LPS 诱导的 Keap-1 表达，但不改变 iNOS 表达。HMFC 还减弱了 UVB 诱导的 p38、c-Jun N-末端激酶（JNK）1/2 和细胞外信号调节激酶（ERK）1/2 的磷酸化，以及人永生化皮肤角质形成细胞 HaCaT 细胞中 c-Jun 的磷酸化。因此，离心的应用适合于生产高浓度 MFC，作为抗炎和抗氧化海洋化妆品的候选材料。
