Pei Yu, Yang Shengtao, Xiao Zhenbang, Zhou Chunxia, Hong Pengzhi, Qian Zhong-Ji
College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Zhanjiang, China.
Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, China.
Front Bioeng Biotechnol. 2021 Nov 18;9:794818. doi: 10.3389/fbioe.2021.794818. eCollection 2021.
, the red algae belonging to Geliaceae , is a traditional edible and industrial alga in China. A sulfated polysaccharide (GNP) is successfully separated from by acid extraction and two-step column chromatography. Chemical analysis showed that the molecular weight of GNP was 25.8 kDa and the monosaccharide composition had the highest galactose content and confirmed the presence and content (16.5%) of sulfate by Fourier transform infrared spectroscopy (FT-IR) spectrometry as well as barium chloride-gelatin methods. In addition, the effect of GNP on lipopolysaccharide (LPS)-induced oxidative stress and inflammation in macrophages was also evaluated. The research results showed that GNP had fairly strong scavenging activities on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical, hydroxyl radical, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and had Fe-chelating ability in a dose-dependent manner. At the same time, it significantly inhibits the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and the production of pro-inflammatory cytokines in RAW 264.7 cells induced by LPS through blocking the mitogen-activated protein kinase (MAPK)/nuclear factor kappa beta (NF-κB) signaling pathway. These results indicate that GNP may be a latent component anti-inflammation in pharmaceutical and functional food industries.
属于江蓠科的红藻是中国一种传统的可食用和用于工业的藻类。通过酸提取和两步柱色谱法成功地从[红藻名称未给出]中分离出一种硫酸化多糖（GNP）。化学分析表明，GNP的分子量为25.8 kDa，单糖组成中半乳糖含量最高，通过傅里叶变换红外光谱（FT-IR）光谱法以及氯化钡-明胶法证实了硫酸盐的存在及含量（16.5%）。此外，还评估了GNP对脂多糖（LPS）诱导的巨噬细胞氧化应激和炎症的影响。研究结果表明，GNP对2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基、羟基自由基和1,1-二苯基-2-苦基肼（DPPH）自由基具有相当强的清除活性，并具有剂量依赖性的铁螯合能力。同时，它通过阻断丝裂原活化蛋白激酶（MAPK）/核因子κB（NF-κB）信号通路，显著抑制LPS诱导的RAW 264.7细胞中诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的表达以及促炎细胞因子的产生。这些结果表明，GNP可能是制药和功能性食品行业中一种潜在的抗炎成分。
