Engineering Research Center of Sichuan Province Higher School of Local Chicken Breeds Industrialization in Southern Sichuan, College of Life Science, Leshan Normal University, Leshan, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Epigenetics. 2023 Dec;18(1):2145058. doi: 10.1080/15592294.2022.2145058. Epub 2022 Nov 15.
The content and distribution of adipocytes is an important factor that affects meat quality. Previous studies showed that circRNAs are involved in various physiological processes. Nevertheless, more research is needed to investigate the function of circRNAs in adipogenesis. The present study examines the effects of circRNF111 on adipogenesis of bovine preadipocyte and aims to elucidate the underlying molecular mechanisms. In our study, the sequence signature of circRNF111 was identified using bioinformatics, RNA-FISH, and sequencing. Mechanistically, knockdown or exogenous expression of circRNF111 in preadipocytes was done to prove the functional significance of circRNF111. Combined with bioinformatics, a dual fluorescein reporter system, and immunoprecipitation, the interaction between circRNF111, miR-27a-3p, and the target gene was verified. The results reveal that circRNF111 is positively correlated with adipocyte differentiation. The newly identified bovine circRNF111 functions as a miR-27a-3p sponge to rescue the inhibitory effect of miR-27a-3p on the gene, thereby promoting adipogenesis.
脂肪细胞的含量和分布是影响肉质的一个重要因素。先前的研究表明，circRNAs 参与了各种生理过程。然而，仍需要更多的研究来探讨 circRNAs 在脂肪生成中的功能。本研究检测了 circRNF111 对牛前体脂肪细胞脂肪生成的影响，旨在阐明其潜在的分子机制。在本研究中，我们使用生物信息学、RNA-FISH 和测序技术鉴定了 circRNF111 的序列特征。在脂肪细胞中敲低或过表达 circRNF111，以证明 circRNF111 的功能意义。结合生物信息学、双荧光素酶报告系统和免疫沉淀技术，验证了 circRNF111、miR-27a-3p 和靶基因之间的相互作用。结果表明，circRNF111 与脂肪细胞分化呈正相关。新鉴定的牛 circRNF111 作为 miR-27a-3p 的海绵，可挽救 miR-27a-3p 对基因的抑制作用，从而促进脂肪生成。
