Department of Thyroid Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China.
Epigenetics. 2023 Dec;18(1):2210339. doi: 10.1080/15592294.2023.2210339.
The circular RNAs (circRNAs) involved in competitive endogenous RNA (ceRNA) mechanism are critical modulators affecting pathogenesis of thyroid carcinoma (TC). The study's goal was to investigate the effects of circ 0003747 on the biological progression of papillary thyroid cancer (PTC). Normal thyroid cells Nthy-ori3-1 and TC derived cell lines were used in our study. Sanger sequencing and RNase R treatment were utilized for validating the circular structure of circ_0003747. In our work, circ_0003747 was found to be highly expressed in TC cells. Circ_0003747 knockdown reduced TC cell viability, proliferation, migration, and invasion while increasing cell apoptosis. Circ_0003747 targeted and negatively regulated miR-338-3p expression. Besides, miR-338-3p interacted with PLCD3 to repress its expression. Overexpression of miR-338-3p inhibited TC cell progression, and PLCD3 reversed these effects. Furthermore, PLCD3 overexpression reversed the effects of circ_0003747 knockdown on TC cells. Additionally, the knockdown of circ_0003747 remarkably suppressed tumour size and growth, restrained PLCD3 expression and promoted miR-338-3p expression in nude mice. In conclusion, circ_0003747 facilitated the biological progression of TC by modulating the miR-338-3p/PLCD3 axis, and it may be a new target for TC treatment. [Figure: see text] TC: Thyroid carcinoma; PTC: Papillary thyroid carcinoma; CircRNAs: Circular RNAs; MiRNA: MicroRNA; EMT: Epithelial-mesenchymal transition; HCC: Hepatocellular carcinoma; PLCD3: Phospholipase C Delta 3; CeRNA: Competitive endogenous RNA.
环状 RNA（circRNAs）参与竞争性内源性 RNA（ceRNA）机制，是影响甲状腺癌（TC）发病机制的关键调节因子。本研究旨在探讨环状 RNA 0003747 对甲状腺乳头状癌（PTC）生物学进展的影响。本研究使用了正常甲状腺细胞 Nthy-ori3-1 和 TC 来源的细胞系。我们利用 Sanger 测序和 RNase R 处理来验证 circ_0003747 的环状结构。在我们的工作中，发现 circ_0003747 在 TC 细胞中高度表达。circ_0003747 敲低降低了 TC 细胞的活力、增殖、迁移和侵袭能力，同时增加了细胞凋亡。circ_0003747 靶向并负调控 miR-338-3p 的表达。此外，miR-338-3p 与 PLCD3 相互作用，抑制其表达。过表达 miR-338-3p 抑制 TC 细胞的进展，而 PLCD3 逆转了这些作用。此外，circ_0003747 敲低对 TC 细胞的作用被 PLCD3 过表达逆转。此外，circ_0003747 的敲低显著抑制裸鼠肿瘤的大小和生长，抑制 PLCD3 的表达并促进 miR-338-3p 的表达。总之，circ_0003747 通过调节 miR-338-3p/PLCD3 轴促进 TC 的生物学进展，它可能是 TC 治疗的新靶点。[图：见正文] TC：甲状腺癌；PTC：甲状腺乳头状癌；circRNAs：环状 RNA；miRNA：微小 RNA；EMT：上皮-间充质转化；HCC：肝细胞癌；PLCD3：磷酯酶 C Delta 3；CeRNA：竞争性内源性 RNA。
