Benítez-Arvizu Gamaliel, Castro-Jácome Tania Patricia, Tovar-Pérez Erik, Alcántara-Quintana Eugenia
Instituto Mexicano del Seguro Social, Centro Médico Nacional Siglo XXI, Hospital de Especialidades "Dr. Bernardo Sepúlveda Gutiérrez", Unidad Complementaria Banco de Sangre. Ciudad de México, México.
Instituto Tecnológico de Tepic, Laboratorio Integral de Investigación en Alimentos. Tepic, Nayarit, México.
Rev Med Inst Mex Seguro Soc. 2023 Jan 1;61(Suppl 1):S4-S11.
Cervical cancer is one of the leading causes of death in women worldwide, both in developed and developing countries. Therefore, effective treatment of cervical cancer with potential anti-tumor drugs is important. However, new treatments inspired by nutritional medicine are needed.
To use the human cervical cancer cell lines HeLa and SiHa to evaluate the antiproliferative, apoptotic, and migratory activity of sorghum (kafirins).
The anticancer effects of the kafirins were examined by counting cells, MTT assays, apoptosis, and migration assays.
This investigation showed that sorghum induced growth inhibition of HeLa and SiHa cells at a significant level. The growth inhibition is dose-dependent and irreversible. When HeLa and SiHa cells were treated with sorghum due to the activity of kafirins, morphological changes were observed, which were identified through the formation of apoptopic bodies. And the kafirins at concentrations of 37.5, 75, 150, and 300 μg/mL decreased the migration of HeLa cells and SiHa cells.
This paper demonstrates the induction of antiproliferative, apoptotic, and anti-migratory activity in HeLa and SiHa cells by kafirins. Sorghum may be used as a nutraceutical with potential cancer-prevention benefits.
宫颈癌是全球女性死亡的主要原因之一，在发达国家和发展中国家均如此。因此，用潜在的抗肿瘤药物有效治疗宫颈癌很重要。然而，需要受营养医学启发的新疗法。
利用人宫颈癌细胞系HeLa和SiHa评估高粱醇溶蛋白的抗增殖、凋亡和迁移活性。
通过细胞计数、MTT法、凋亡检测和迁移检测来研究高粱醇溶蛋白的抗癌作用。
本研究表明，高粱能显著抑制HeLa和SiHa细胞的生长。生长抑制呈剂量依赖性且不可逆。当HeLa和SiHa细胞因高粱醇溶蛋白的活性而用高粱处理时，观察到形态学变化，通过凋亡小体的形成得以确认。并且浓度为37.5、75、150和300μg/mL的高粱醇溶蛋白可降低HeLa细胞和SiHa细胞的迁移。
本文证明了高粱醇溶蛋白可诱导HeLa和SiHa细胞产生抗增殖、凋亡和抗迁移活性。高粱可用作具有潜在防癌益处的营养保健品。
