艾灸足三里（ST36）和肾俞（BL23）对佐剂性关节炎兔巨噬细胞移动抑制因子和巨噬细胞凋亡表达的影响。
Efficacy of Moxa-burning heat stimulating Zusanli (ST36) and Shenshu (BL23) on expressions of macrophage migration inhibitory factor and macrophage apoptosis in rabbits with adjuvant-induced arthritis.
机构信息
School of Acupuncture-Moxibustion and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
Pain Department, Chengdu First People's Hospital/ Chengdu Integrated TCM & Western Medicine Hospital, Chengdu 610095, China.
出版信息
J Tradit Chin Med. 2022 Dec;42(6):980-987. doi: 10.19852/j.cnki.jtcm.20220817.001.
OBJECTIVE
To evaluate the effects of moxa-burning heat stimulating acupoints Zusanli (ST36) and Shenshu (BL23) on macrophage migration inhibitory factor (MIF) and its related molecules which can provide scientific experimental basis for the clinical application of moxibustion treatment of rheumatoid arthritis (RA).
METHODS
Thirty rabbits were randomly assigned to control group, RA model (established by injecting Freund's Complete Adjuvant) group (RA group) and RA model with moxibustion group [Moxa group, Zusanli (ST36) and Shenshu (BL23), 5 moxa pillars/day, 6 d × 3]. The expressions of MIF mRNA were evaluated with reverse transcription polymerase chain reaction; the apoptosis rates of macrophages were detected by erminal deoxynucleotidyl transferase-mediated dTUP nick end labeling; the expressions of related signal molecules were detected with immunohistochemical S-P method and the levels of IL-2 were detected with enzyme-linked immunosorbent assay.
RESULTS
The expressions of MIF mRNA, extracellular regulated protein kinases 2, p38 mitogen-activated protein kinase and nuclear factor-κ-gene binding p65 in synovial tissue of RA group were significantly increased when compared with control group, which were lower remarkably in moxa group than those in RA group. The apoptosis rates of macrophages in RA group were significantly down-regulated as compared with the control group, which were up-regulated in moxa group compared with the RA group. The levels of IL-2 in synovial fluid from the RA group were elevated significantly as compared with that from control group, but those of the moxa group were reduced when compared with those from RA group.
CONCLUSIONS
Moxibustion may simultaneously regulate the expressions of MIF and its related signaling pathways molecules, the apoptosis rate of macrophages in synovial tissue, as well as the level of inflammatory factors in synovial fluid. The results suggest that the anti-inflammatory effect of moxibustion on RA may be related to inhibit the expression of MIF in synovial tissue, the molecules of some related signaling pathways and promote the apoptosis of macrophage.
目的
评价艾灸足三里（ST36）和肾俞（BL23）穴对巨噬细胞移动抑制因子（MIF）及其相关分子的影响，为艾灸治疗类风湿关节炎（RA）的临床应用提供科学的实验依据。
方法
30 只家兔随机分为对照组、RA 模型（弗氏完全佐剂注射）组（RA 组）和艾灸组[艾灸组，足三里（ST36）和肾俞（BL23），每天 5 柱艾灸，6d×3]。采用逆转录聚合酶链反应法检测 MIFmRNA 的表达；末端脱氧核苷酸转移酶介导的 dTUP 缺口末端标记法检测巨噬细胞凋亡率；免疫组织化学 S-P 法检测相关信号分子的表达，酶联免疫吸附试验检测 IL-2 水平。
结果
与对照组相比，RA 组滑膜组织中 MIFmRNA、细胞外调节蛋白激酶 2、p38 丝裂原活化蛋白激酶和核因子-κB 基因结合 p65 的表达显著增加，艾灸组明显低于 RA 组。与对照组相比，RA 组滑膜组织中巨噬细胞的凋亡率明显下调，艾灸组明显上调。与对照组相比，RA 组关节滑液中 IL-2 水平明显升高，艾灸组明显降低。
结论
艾灸可能通过调节滑膜组织中 MIF 及其相关信号通路分子、滑膜组织中巨噬细胞的凋亡率以及关节滑液中炎症因子的水平，同时发挥作用。结果提示艾灸对 RA 的抗炎作用可能与抑制滑膜组织中 MIF 的表达、某些相关信号通路分子以及促进巨噬细胞凋亡有关。
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