Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia.
Vector Borne Disease Sector, Disease Control Division, Ministry of Health Malaysia, Putrajaya, Malaysia.
Parasitol Res. 2023 Jan;122(1):195-200. doi: 10.1007/s00436-022-07716-z. Epub 2022 Nov 15.
Plasmodium knowlesi is a simian malaria parasite that causes significant zoonotic infections in Southeast Asia, particularly in Malaysia. The Plasmodium thrombospondin-related apical merozoite protein (TRAMP) plays an essential role in the invasion of the parasite into its host erythrocyte. The present study investigated the genetic polymorphism and natural selection of the full length PkTRAMP from P. knowlesi clinical isolates from Malaysia. Blood samples (n = 40) were collected from P. knowlesi malaria patients from Peninsular Malaysia and Malaysian Borneo. The PkTRAMP gene was amplified using PCR, followed by cloning into a plasmid vector and sequenced. Results showed that the nucleotide diversity of PkTRAMP was low (π: 0.009). Z-test results indicated negative (purifying) selection of PkTRAMP. The alignment of the deduced amino acid sequences of PkTRAMP of Peninsular Malaysia and Malaysian Borneo revealed 38 dimorphic sites. A total of 27 haplotypes were identified from the amino acid sequence alignment. Haplotype analysis revealed that there was no clustering of PkTRAMP from Peninsular Malaysia and Malaysian Borneo.
食蟹猴疟原虫是一种灵长类疟原虫，在东南亚地区，特别是马来西亚，会导致严重的人畜共患感染。疟原虫血小板反应蛋白相关顶质膜蛋白（TRAMP）在寄生虫侵入宿主红细胞的过程中发挥着重要作用。本研究调查了来自马来西亚的食蟹猴疟原虫临床分离株全长 PkTRAMP 的遗传多态性和自然选择。从马来半岛和婆罗洲的马来西亚疟疾病人采集了 40 份血样。使用 PCR 扩增 PkTRAMP 基因，然后克隆到质粒载体并进行测序。结果表明，PkTRAMP 的核苷酸多样性较低（π：0.009）。Z 检验结果表明 PkTRAMP 受到负向（净化）选择。对马来半岛和婆罗洲马来西亚的 PkTRAMP 推导氨基酸序列进行比对，发现 38 个二态性位点。从氨基酸序列比对中鉴定出 27 种单倍型。单倍型分析表明，来自马来半岛和婆罗洲马来西亚的 PkTRAMP 没有聚类。
