Chaudhari Diptaraj, Kiran Shashi, Choudhary Ashish, Silveira Kris, Narwade Nitin, Dhotre Dhiraj, Khazir Jabeena, Mir Bilal Ahmad, Shouche Yogesh S, Rahi Praveen
National Center for Microbial Resource, National Center for Cell Science, Central Tower, Sai Trinity Building, Second Floor, Pashan, Pune, Maharashtra, 411021, India.
Present address: USF Center for Microbiome Research, University of South Florida, Tampa, USA.
Int Microbiol. 2023 May;26(2):257-267. doi: 10.1007/s10123-022-00297-y. Epub 2022 Nov 15.
Indian lotus (Nelumbo nucifera) is one of the dominant aquatic plants cultivated in Dal Lake, situated at 1586 m above mean sea level (MSL) in the northeast of Srinagar, Kashmir. Despite their economic and ecological role, the microbial communities associated with the lotus plant are still unexplored. In this study, we investigated the prokaryotic communities on surfaces of different lotus microhabitats (roots, rhizome, leaves, flowers, and fruits), lake water, and sediments using 16S rRNA gene amplicon sequencing. Overall, prokaryotic diversity decreased significantly on the surface of lotus microhabitats in comparison to the lake water and sediments. Among the microhabitats of lotus, roots and leaves harbored more diverse communities in comparison to rhizomes, fruits, and flowers. A total of 98 genera were shared by lotus and the Dal Lake sediments and water. However, significant differences were found in their relative abundance; for example, Pseudomonas was the most dominant genus on the majority of lotus microhabitats. On the other hand, Flavobacterium was highly abundant in the lake water, while a higher abundance of Acinetobacter was recorded in sediments. Additionally, we also noted the presence of potential human pathogenic genera including Escherichia-Shigella, Enterobacter, Pantoea, Raoultella, Serratia, and Sphingomonas on the lotus microhabitats. Predicted functions of prokaryotic communities revealed a higher abundance of genes associated with nutrient uptake in the microhabitats of the lotus. This study offered first-hand information on the prokaryotic communities harbored by lotus plants and water and sediments of the Dal Lake and demonstrated the adaptation of diverse communities to microhabitats of lotus.
印度莲（Nelumbo nucifera）是克什米尔地区斯利那加东北部海拔1586米的达尔湖种植的主要水生植物之一。尽管其具有经济和生态作用，但与莲相关的微生物群落仍未得到充分研究。在本研究中，我们利用16S rRNA基因扩增子测序技术，对不同莲微生境（根、根茎、叶、花和果实）表面、湖水和沉积物中的原核生物群落进行了调查。总体而言，与湖水和沉积物相比，莲微生境表面的原核生物多样性显著降低。在莲的微生境中，根和叶比根茎、果实和花拥有更多样化的群落。莲与达尔湖沉积物和水中共有98个属。然而，它们的相对丰度存在显著差异；例如，假单胞菌是大多数莲微生境中最主要的属。另一方面，黄杆菌在湖水中含量很高，而不动杆菌在沉积物中的丰度更高。此外，我们还注意到莲微生境上存在潜在的人类致病属，包括埃希氏菌-志贺氏菌属、肠杆菌属、泛菌属、拉乌尔菌属、沙雷氏菌属和鞘氨醇单胞菌属。原核生物群落的预测功能显示，莲微生境中与养分吸收相关的基因丰度更高。本研究提供了关于莲植物以及达尔湖的水和沉积物中所含原核生物群落的第一手信息，并证明了不同群落对莲微生境的适应性。
