Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland.
Institute of Physiology, University of Bonn, Bonn, Germany.
Methods Mol Biol. 2023;2596:291-302. doi: 10.1007/978-1-0716-2831-7_20.
The biochemical and cell biological profiling of contractile fiber types and subcellular structures plays a central role in basic and applied myology. Mass spectrometry-based proteomics presents an ideal approach for the systematic identification of proteomic and subproteomic markers. These representative components of fast versus slow muscle fibers and their subcellular fractions are highly useful for in-depth surveys of skeletal muscle adaptations to physiological challenges, as well as the improvement of diagnostic, prognostic, and therapy-monitoring methodologies in muscle pathology. This chapter outlines the identification of subproteomic markers for skeletal muscle profiling based on bottom-up and top-down approaches, including fluorescence two-dimensional difference gel electrophoresis (2D-DIGE).
收缩纤维类型和亚细胞结构的生化和细胞生物学特征分析在基础和应用肌科学中起着核心作用。基于质谱的蛋白质组学为系统鉴定蛋白质组学和亚蛋白质组学标志物提供了一种理想的方法。这些快肌纤维和慢肌纤维及其亚细胞成分的代表性成分对于深入研究骨骼肌对生理挑战的适应性非常有用，也有助于改善肌肉病理学中的诊断、预后和治疗监测方法。本章概述了基于自上而下和自下而上方法（包括荧光二维差异凝胶电泳（2D-DIGE））鉴定骨骼肌分析的亚蛋白质组学标志物。
