Plant Genetics and Rhizosphere Processes Laboratory, TERRA Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.
Plant Genetics and Rhizosphere Processes Laboratory, TERRA Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.
Trends Plant Sci. 2023 Mar;28(3):297-311. doi: 10.1016/j.tplants.2022.10.005. Epub 2022 Nov 12.
Recent metagenomic studies which focused on virus characterization in the entire plant environment have revealed a remarkable viral diversity in plants. The exponential discovery of viruses also requires the concomitant implementation of high-throughput methods to perform their functional characterization. Despite several limitations, the development of viral infectious clones remains a method of choice to understand virus biology, their role in the phytobiome, and plant resilience. Here, we review the latest approaches for efficient characterization of plant viruses and technical advances built on high-throughput sequencing and synthetic biology to streamline assembly of viral infectious clones. We then discuss the applications of plant viral vectors in fundamental and applied plant research as well as their technical and regulatory limitations, and we propose strategies for their safer field applications.
最近的宏基因组研究集中于在整个植物环境中对病毒进行特征描述，揭示了植物中存在显著的病毒多样性。病毒的指数级发现也需要同时实施高通量方法来进行它们的功能特征描述。尽管存在一些限制，但是病毒感染性克隆的开发仍然是理解病毒生物学、它们在植物微生物组中的作用以及植物弹性的首选方法。在这里，我们综述了用于有效鉴定植物病毒的最新方法，以及基于高通量测序和合成生物学的技术进展，以简化病毒感染性克隆的组装。然后，我们讨论了植物病毒载体在基础和应用植物研究中的应用，以及它们的技术和监管限制，并提出了它们在田间更安全应用的策略。
