Thongnak Laongdao, Jaruan Onanong, Pengrattanachot Nattavadee, Promsan Sasivimon, Phengpol Nichakorn, Sutthasupha Prempree, Jaikumkao Krit, Sriyotai Woraprapa, Mahatheeranont Sugunya, Lungkaphin Anusorn
Renal Transporters and Molecular Signaling Unit, Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok, Thailand.
Phytother Res. 2023 Mar;37(3):935-948. doi: 10.1002/ptr.7675. Epub 2022 Nov 15.
It has recently been reported that black rice (BR) extract has anti-obesity, anti-diabetic, and anti-osteoporosis effects. It has been shown to reduce obese-related kidney dysfunction in animal models. This study aimed to investigate the effect of resistant starch from BR (RS) on renal inflammation, oxidative stress, and apoptosis in obese insulin resistant rats. Male Wistar rats were divided into six groups: normal diet (ND), ND treated with 150 mg of RS (NDRS150), high-fat (HF) diet, HF treated with 100 and 150 mg of RS (HFRS100), (HFRS150), and HF treated with metformin as a positive control. Insulin resistance was shown in the HF rats by glucose intolerance, increased insulin, total area under the curve of glucose and homeostasis model assessment of insulin resistance and dyslipidemia. The resulting metabolic disturbance in the HF rats caused renal inflammation, fibrosis and apoptosis progressing to kidney injury and dysfunction. Prebiotic RS including anthocyanin from BR at doses of 100 and 150 mg ameliorated insulin resistance, dyslipidemia and liver injury. Treatment with RS reduced TGF-β fibrotic and apoptotic pathways by inhibition of NF-κB and inflammatory cytokines which potentially restore kidney damage and dysfunction. In conclusion, prebiotic RS from BR ameliorated obesity induced renal injury and dysfunction by attenuating inflammatory, fibrotic, and apoptotic pathways in insulin resistant rats induced by HF.
最近有报道称，黑米（BR）提取物具有抗肥胖、抗糖尿病和抗骨质疏松作用。在动物模型中，它已被证明可减轻肥胖相关的肾功能障碍。本研究旨在探讨黑米抗性淀粉（RS）对肥胖胰岛素抵抗大鼠肾脏炎症、氧化应激和细胞凋亡的影响。雄性Wistar大鼠分为六组：正常饮食（ND）组、用150毫克RS处理的ND组（NDRS150）、高脂（HF）饮食组、用100毫克和150毫克RS处理的HF组（HFRS100、HFRS150），以及用二甲双胍作为阳性对照处理的HF组。HF大鼠表现出胰岛素抵抗，包括葡萄糖耐量异常、胰岛素增加、葡萄糖曲线下总面积以及胰岛素抵抗和血脂异常的稳态模型评估。HF大鼠由此产生的代谢紊乱导致肾脏炎症、纤维化和细胞凋亡，进而发展为肾损伤和功能障碍。剂量为100毫克和150毫克的含黑米花青素的益生元RS改善了胰岛素抵抗、血脂异常和肝损伤。RS处理通过抑制NF-κB和炎性细胞因子减少了TGF-β纤维化和凋亡途径，这可能恢复肾脏损伤和功能障碍。总之，黑米中的益生元RS通过减轻HF诱导的胰岛素抵抗大鼠的炎症、纤维化和凋亡途径，改善了肥胖诱导的肾损伤和功能障碍。
