Xiaoyun Guo, Hongjun Li, Cuijing Meng, Li Rong, Mei Zhang
Department of Nephrology, Second Hospital of Tianjin Medical University, Tianjin, China.
Department of Infection Immunity, Second Hospital of Tianjin Medical University, Tianjin, China.
Medicine (Baltimore). 2025 Jan 3;104(1):e41202. doi: 10.1097/MD.0000000000041202.
Chronic kidney disease significantly affects human health by loss of excretory kidney function. MicroRNAs have potential predictive and therapeutic significance for chronic kidney disease and fibrosis-related kidney diseases. This study aimed to investigate expression profiling and clinical significance of microRNA-204 (miR-204) expression in patients with chronic kidney disease.
A total of 126 patients with chronic kidney disease and age-matched 126 healthy controls were enrolled in this study. Blood samples were collected from participants and expression levels of miR-204 were detected using reverse transcription quantitative polymerase chain reaction. Expression of inflammatory cytokines in glomerular cells was measured using reverse transcription quantitative polymerase chain reaction. Inflammatory cytokines in serum were analyzed using enzyme-linked immunosorbent assay in all participants. Multivariate Cox-regression analysis was used to analyze the association between serum level of miR-204 and inflammation, renal fibrosis, and degree of chronic kidney disease.
Chronic kidney disease patients had higher inflammatory cytokines including IL-1β, IL-6, TNF-α, IL-10, and IL-17 than healthy volunteers. Expression levels of inflammatory cytokines (IL-1β, IL-6, TNF-α, IL-10, and IL-17) were upregulated in patients with chronic kidney disease compared to healthy volunteers. Serum level of miR-204 was lower in chronic kidney disease patients than healthy patients. Expression of miR-204 was higher in healthy volunteers than patients with chronic kidney disease. In addition, expression of miR-204 was lower in glomerular cells in chronic kidney disease patients than those in the healthy volunteers. Furthermore, higher serum level of miR-204 was associated with better renal function in chronic kidney disease patients than patients who had lower serum level of miR-204. High serum levels of miR-204 were associated with degree of renal fibrosis and injury of chronic kidney disease patients. Multivariate Cox-regression analysis identified expression of miR-204 was positively correlated with inflammation in patients with chronic kidney disease.
Outcomes indicate that serum levels of miR-204 are downregulated in serum in patients with chronic kidney disease. Data suggest that serum levels of miR-204 can be used to evaluate the renal function in patients with chronic kidney disease.
慢性肾脏病通过肾功能丧失显著影响人类健康。微小RNA对慢性肾脏病及纤维化相关肾脏疾病具有潜在的预测和治疗意义。本研究旨在探讨慢性肾脏病患者中微小RNA-204（miR-204）的表达谱及其临床意义。
本研究共纳入126例慢性肾脏病患者及126例年龄匹配的健康对照。采集参与者的血样，采用逆转录定量聚合酶链反应检测miR-204的表达水平。采用逆转录定量聚合酶链反应检测肾小球细胞中炎性细胞因子的表达。所有参与者均采用酶联免疫吸附测定法分析血清中的炎性细胞因子。采用多因素Cox回归分析血清miR-204水平与炎症、肾纤维化及慢性肾脏病程度之间的关联。
慢性肾脏病患者的炎性细胞因子包括白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）和白细胞介素-17高于健康志愿者。与健康志愿者相比，慢性肾脏病患者炎性细胞因子（IL-1β、IL-6、TNF-α、IL-10和IL-17）的表达水平上调。慢性肾脏病患者的血清miR-204水平低于健康患者。健康志愿者中miR-204的表达高于慢性肾脏病患者。此外，慢性肾脏病患者肾小球细胞中miR-204的表达低于健康志愿者。此外，血清miR-204水平较高的慢性肾脏病患者的肾功能优于血清miR-204水平较低的患者。血清miR-204水平高与慢性肾脏病患者的肾纤维化程度及损伤相关。多因素Cox回归分析确定miR-204的表达与慢性肾脏病患者的炎症呈正相关。
结果表明慢性肾脏病患者血清中miR-204水平下调。数据表明血清miR-204水平可用于评估慢性肾脏病患者的肾功能。
