Key Laboratory of Applied Surface and Colloid Chemistry of Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an710062, P. R. China.
State Key Laboratory of Innovative Medicine and High Efficiency and Energy Saving Pharmaceutical Equipment and Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang330004, P. R. China.
ACS Appl Mater Interfaces. 2022 Nov 30;14(47):53323-53330. doi: 10.1021/acsami.2c16469. Epub 2022 Nov 16.
Promising techniques for detecting and quantifying active components in the plants and foods have received global concern in smart agriculture. Dual-mode optical assays are becoming more attractive and popular thanks to robust and reliable analysis parameters. We herein unveil a novel turn-on and dual-mode sensor array comprising three kinds of reactive indicators including ring-closed rhodamine-hydrazine, squaraine-hydrazine, and 2,4-dinitrophenylhydrazine for evaluating perillaldehyde. Significant colorimetric and fluorescent changes were triggered through reacting primary amine/hydrazine with the active aldehyde group in perillaldehyde, thus turning on the chromogenic responses of all the indicators. Optimal colorimetric sensing showed good responses to perillaldehyde ranged up to 100 mM in ethanol. Dramatic fluorescence enhancement was also exhibited, illustrating good selectivity as well as high sensitivity (detection limit ∼20.0 μM). Inspired by rapid chemical reactions and distinct optical changes, distinct sensor array strips loaded with the optimal solid-state reactive indicators were developed for evaluating the perillaldehyde content in the perilla frutescence leaves. Smartphone-enabled readout system and digital data processing were further performed for chemometric analysis. A good correlation was obtained and the semiquantitative evaluation of the perillaldehyde content could be achieved within 15 min, possessing the significant features of naked-eye recognition, easy operation, and disposability. To the best of our knowledge, present work demonstrated the use of chromogenic sensing strips to evaluate the active perillaldehyde content in solution and vapor phases for the first time. Taken together, these characteristics also indicate that the present turn-on sensor array has great potential applications in the precise detection and evaluation of perillaldehyde in the forthcoming smart agriculture.
在智慧农业中，用于检测和定量植物和食品中活性成分的有前景的技术受到了全球关注。由于具有稳健可靠的分析参数，双模式光学测定法变得更具吸引力和普及性。在此，我们揭示了一种新颖的开启型和双模式传感器阵列，该传感器阵列由包括闭环罗丹明-腙、方酸-腙和 2,4-二硝基苯腙在内的三种反应性指示剂组成，用于评估紫苏醛。通过与紫苏醛中的活性醛基反应，伯胺/腙引发了显著的比色和荧光变化，从而开启了所有指示剂的显色响应。最佳比色传感在乙醇中对紫苏醛的响应高达 100 mM。还表现出了显著的荧光增强，显示出良好的选择性以及高灵敏度（检测限约为 20.0 μM）。受快速化学反应和明显光学变化的启发，开发了加载最佳固态反应性指示剂的独特传感器阵列条，用于评估紫苏叶中的紫苏醛含量。进一步进行了基于智能手机的读取系统和数字数据处理，以进行化学计量学分析。获得了良好的相关性，可以在 15 分钟内实现紫苏醛含量的半定量评估，具有肉眼识别、易于操作和可处置的显著特点。据我们所知，目前的工作首次展示了使用显色传感条来评估溶液和蒸气相中紫苏醛的活性含量。总的来说，这些特点还表明，该新型开启型传感器阵列在未来的智慧农业中具有对紫苏醛进行精确检测和评估的巨大应用潜力。
