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中国胡颓子果实(EAF)中果糖和葡萄糖共发酵生产乙醇的方法。

Co-fermentation Approach of Fructose and Glucose to Ethanol from Chinese Elaeagnus angustifolia Fruit (EAF).

机构信息

Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, No. 159 Longpan Road, Nanjing, 210037, People's Republic of China.

Jiangsu Province Key Laboratory of Green Biomass-Based Fuels and Chemicals, Nanjing, 210037, People's Republic of China.

出版信息

Appl Biochem Biotechnol. 2023 Mar;195(3):1770-1780. doi: 10.1007/s12010-022-04213-y. Epub 2022 Nov 17.

Abstract

The soluble and fermentable carbohydrate contents was detected over 47% of glucose and fructose in Chinese Elaeagnus angustifolia fruit powder (EAF), being over 47 wt% sugar content more than that of grape. Ethanol was therefore fermented directly from EAF, and different submerged fermentation modes were comparatively employed to optimize ethanol harvest. The results indicated that glucose has certain competitive inhibition on fructose bio-utilization, as well as the EAF solid residue involved fermentation mode also hindered the fermented-ethanol titer. Pectinase addition and in situ hydrolysis seemed to assist somewhat the fermentation. The water-solute fermentation mode is preferable, and glucose and fructose components were completely consumed and converted to 80.96 g/L ethanol at 87.6% ethanol yield even under tannin and pectin inhibition. The fermentation result could provide some experimental data and an approach to not only new biomass resource explores of bioethanol and alcohol beverage production, but also the technological development on valorization commercials of EAF in global draught areas.

摘要

中国胡颓子果粉(EAF)中可溶和可发酵碳水化合物含量检测出葡萄糖和果糖含量超过 47%,含糖量超过 47wt%,比葡萄还高。因此,乙醇可直接从 EAF 发酵而来,采用不同的深层发酵方式来优化乙醇产量。结果表明,葡萄糖对果糖的生物利用有一定的竞争抑制作用,EAF 固体残渣参与发酵模式也会阻碍发酵-乙醇的产量。果胶酶的添加和原位水解似乎对发酵有一定的帮助。水溶剂发酵模式是优选的,即使在单宁和果胶的抑制下,葡萄糖和果糖成分也能完全消耗,并转化为 80.96g/L 乙醇,乙醇得率达到 87.6%。发酵结果不仅为生物乙醇和酒精饮料生产的新生物质资源探索提供了一些实验数据和方法,而且为全球干旱地区 EAF 的商业增值技术发展提供了一些实验数据和方法。

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