Lee Ok Kyung, Oh You-Kwan, Lee Eun Yeol
Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea.
Clean Fuel Department, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
Bioresour Technol. 2015 Nov;196:22-7. doi: 10.1016/j.biortech.2015.07.040. Epub 2015 Jul 20.
The residual biomass of Chlorella sp. KR-1 obtained after lipid extraction was used for saccharification and bioethanol production. The carbohydrate was saccharified using simple enzymatic and chemical methods using Pectinex at pH 5.5 and 45°C and 0.3N HCl at 121°C for 15min with 76.9% and 98.2% yield, respectively, without any pretreatment. The residual biomass contained 49.7% carbohydrate consisting of 82.4% fermentable sugar and 17.6% non-fermentable sugar, which is valuable for bioethanol fermentation. Approximately 98.2% of the total carbohydrate was converted into monosaccharide (fermentable+non-fermentable sugar) using dilute acid saccharification. The fermentable sugar was subsequently fermented to bioethanol through separate hydrolysis and fermentation with a fermentation yield of 79.3%. Overall, 0.4g ethanol/g fermentable sugar and 0.16g ethanol/g residual biomass were produced.
经脂质提取后获得的小球藻KR-1残余生物质用于糖化和生物乙醇生产。使用简单的酶法和化学方法进行碳水化合物糖化，在pH 5.5和45°C条件下使用果胶酶，以及在121°C条件下使用0.3N盐酸处理15分钟，糖化产率分别为76.9%和98.2%，且无需任何预处理。残余生物质含有49.7%的碳水化合物，其中82.4%为可发酵糖，17.6%为不可发酵糖，这对于生物乙醇发酵很有价值。使用稀酸糖化法，约98.2%的总碳水化合物被转化为单糖（可发酵糖+不可发酵糖）。随后，可发酵糖通过单独水解和发酵转化为生物乙醇，发酵产率为79.3%。总体而言，每克可发酵糖产生0.4克乙醇，每克残余生物质产生0.16克乙醇。
