Zhang Jing, Shen Jinhui, Cong Qian, Martin Geoff, Grishin Nick V
1. Departments of Biophysics, Biochemistry.
2. Eugene McDermott Center For Human Growth & Development, University of Texas Southwestern Medical Center.
Trop Lepid Res. 2022 Dec;32(2):73-78. doi: 10.5281/zenodo.7246139. Epub 2022 Oct 29.
We obtained whole genome shotgun sequence reads for a number of Firetip skippers (subfamily Pyrrhopyginae), including all known species from the genera Watson, 1893 and Mielke, 2002 and representative species of Hübner, [1819]. Phylogenetic analysis of their protein-coding regions unexpectedly revealed that Bell, 1931 was not monophyletic with the other species of (type species Hewitson, 1870). Instead, formed a phylogenetic lineage as ancient as other three genera in its clade (, and ) that rapidly diversified from their ancestor. Therefore a new genus, Grishin, . (type species ), is proposed for this lineage. The specimen that we sequenced was the . holotype in the Natural History Museum, London, leaving no doubt that we are dealing with this species. Genomic sequencing and comparison of specimens from museum collections offers a powerful strategy to reveal unforeseen phylogenetic relationships, and sequencing of primary types ensures that the conclusions are accurate in terms of nomenclature.
我们获得了一些萤光弄蝶（Pyrrhopyginae亚科）的全基因组鸟枪法测序读数，包括1893年沃森属和2002年迈尔克属的所有已知物种以及[1819年]许布纳属的代表性物种。对它们蛋白质编码区的系统发育分析意外地发现，1931年的贝尔弄蝶属与（模式种为1870年的休伊特弄蝶）的其他物种并非单系群。相反，贝尔弄蝶属在其分支（沃森属、迈尔克属和许布纳属）中形成了一个与其他三个属一样古老的系统发育谱系，该谱系从其祖先迅速分化出来。因此，为这个谱系提出了一个新属，即2024年的格里申弄蝶属（模式种为贝尔弄蝶）。我们测序的标本是伦敦自然历史博物馆的贝尔弄蝶全模式标本，毫无疑问我们研究的就是这个物种。对博物馆收藏标本进行基因组测序和比较提供了一种强大的策略来揭示意外的系统发育关系，对正模标本进行测序确保了结论在命名方面的准确性。
