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小窝蛋白依赖的内吞作用介导了卵巢癌细胞系中碲化镉量子点的细胞摄取。

Caveolae-dependent endocytosis mediates the cellular uptake of CdTe quantum dots in ovarian cancer cell lines.

作者信息

Asadian Zahra, Zare Hakimeh, Aghaei Mahmoud, Panjehpour Mojtaba

机构信息

Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.

Department of Physics, Yazd University, Yazd, I.R. Iran.

出版信息

Res Pharm Sci. 2022 Sep 8;17(5):527-539. doi: 10.4103/1735-5362.355211. eCollection 2022 Oct.

DOI:10.4103/1735-5362.355211
PMID:36386487
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9661687/
Abstract

BACKGROUND AND PURPOSE

Quantum dots (QDs) are semiconductor nanocrystals that are widely used in biology due to their good optical properties. QDs, especially cadmium-based QDs, play an important role in the diagnosis and treatment of cancer due to their intrinsic fluorescence. The aim of the present study was the evaluation of the cellular uptake mechanisms of CdTe QDs in ovarian cancer cell lines.

EXPERIMENTAL APPROACH

In this study, we used CdTe QDs coated with thioglycolic acid. The ovarian cancer cell lines SKOV3 and OVCAR3 were treated with different concentrations of QDs, triamterene, chlorpromazine, and nystatin, and cell viability was evaluated through the MTT test. To find the way of cellular uptake of CdTe QDs, we used the MTT test and interfering compounds in endocytic pathways. Intrinsic fluorescence and cellular internalization of CdTe QDs were assessed using flow cytometry and fluorescence microscopy imaging.

FINDINGS / RESULTS: The viability of CdTe QDs-treated cells dose-dependently decreased in comparison to untreated cells. To evaluate the cellular uptake pathways of CdTe QDs, in most cases, a significant difference was observed when the cells were pretreated with nystatin. The results of flow cytometry showed the cellular uptake of CdTe QDs was dose- and time-dependent.

CONCLUSION AND IMPLICATIONS

Nystatin had a measurable effect on the cellular uptake of CdTe QDs. This finding suggests that caveola-mediated endocytosis has a large portion on the internalization of CdTe QDs. According to the results of this study, CdTe QDs may have potential applications in cancer research and diagnosis.

摘要

背景与目的

量子点（QDs）是半导体纳米晶体，因其良好的光学性质而被广泛应用于生物学领域。量子点，尤其是基于镉的量子点，由于其固有荧光，在癌症的诊断和治疗中发挥着重要作用。本研究的目的是评估碲化镉量子点在卵巢癌细胞系中的细胞摄取机制。

实验方法

在本研究中，我们使用了巯基乙酸包覆的碲化镉量子点。用不同浓度的量子点、氨苯蝶啶、氯丙嗪和制霉菌素处理卵巢癌细胞系SKOV3和OVCAR3，并通过MTT试验评估细胞活力。为了找到碲化镉量子点的细胞摄取方式，我们使用MTT试验和内吞途径中的干扰化合物。使用流式细胞术和荧光显微镜成像评估碲化镉量子点的固有荧光和细胞内化。

研究结果

与未处理的细胞相比，经碲化镉量子点处理的细胞活力呈剂量依赖性下降。为了评估碲化镉量子点的细胞摄取途径，在大多数情况下，当用制霉菌素预处理细胞时，观察到显著差异。流式细胞术结果显示，碲化镉量子点的细胞摄取具有剂量和时间依赖性。

结论与意义

制霉菌素对碲化镉量子点的细胞摄取有显著影响。这一发现表明，小窝介导的内吞作用在碲化镉量子点的内化中占很大比例。根据本研究结果，碲化镉量子点在癌症研究和诊断中可能具有潜在应用。

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