Teng Li, Chen Sen, Hu Zuquan, Chen Jili, Liu Hongmei, Zhang Tingting
Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, Engineering Research Center of Cellular Immunotherapy of Guizhou Province, School of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China.
Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, China.
Front Microbiol. 2022 Oct 26;13:1008409. doi: 10.3389/fmicb.2022.1008409. eCollection 2022.
is an important fungal species owing to its ubiquity in soils, plants, air, and food. In this study, we identified a novel six-segmented polymycovirus, polymycovirus 1 (TaPmV-1). Each of the double-stranded (ds) RNA segments of TaPmV-1 contained a single open reading frame, and the proteins encoded by dsRNA1, dsRNA2, dsRNA3, and dsRNA 5 shared significant amino acid identities of 56, 40, 47, and 43%, respectively, with the corresponding proteins of polymycovirus-1(AfuPmV-1). DsRNA1, dsRNA3, and dsRNA5 of TaPmV-1 encoded an RNA-dependent RNA polymerase (RdRp), a viral methyltransferase, and a PAS-rich protein, respectively. The functions of the proteins encoded by dsRNA2, dsRNA4, and dsRNA6 have not been elucidated. Comparison of the virus-infected strain LSH3 with virus-cured strain LSHVF revealed that infection with TaPmV-l may reduce the production of red pigments and induce the clustering of fungal sclerotia. Furthermore, transcriptomic analyses demonstrated that infection with TaPmV-l downregulated the expression of transcripts related to metabolism, and may correlate with the reduced production of red pigments and clustering of sclerotia in . These results of this study provide novel insights into the mechanism of fungal gene regulation by polymycovirus infections at the transcriptome level, and this study is the first to report a novel polymycovirus of .
由于其在土壤、植物、空气和食物中广泛存在，是一种重要的真菌物种。在本研究中，我们鉴定出一种新型的六片段多分体病毒，即多分体病毒1（TaPmV-1）。TaPmV-1的每个双链（ds）RNA片段都包含一个单一的开放阅读框，并且dsRNA1、dsRNA2、dsRNA3和dsRNA 5编码的蛋白质分别与多分体病毒-1（AfuPmV-1）的相应蛋白质具有56%、40%、47%和43%的显著氨基酸同一性。TaPmV-1的dsRNA1、dsRNA3和dsRNA5分别编码一种RNA依赖性RNA聚合酶（RdRp）、一种病毒甲基转移酶和一种富含PAS的蛋白质。dsRNA2、dsRNA4和dsRNA6编码的蛋白质的功能尚未阐明。将病毒感染菌株LSH3与病毒清除菌株LSHVF进行比较，发现感染TaPmV-1可能会减少红色色素的产生并诱导真菌菌核聚集。此外，转录组分析表明，感染TaPmV-1会下调与代谢相关的转录本的表达，这可能与红色色素产生减少和菌核聚集中的情况相关。本研究的这些结果为转录组水平上多分体病毒感染对真菌基因调控机制提供了新的见解，并且本研究首次报道了一种新型的多分体病毒。
