Wastag Maksymilian, Bieżuńska-Kusiak Katarzyna, Szewczyk Anna, Szlasa Wojciech, Grimling Bożena, Kulbacka Julita
Faculty of Pharmacy, Wroclaw Medical University, Borowska 211A, 50-556, Wroclaw, Poland.
Department of Molecular and Cellular Biology, Wroclaw Medical University, Borowska 211A 50-556, Wroclaw, Poland.
Saudi Pharm J. 2022 Oct;30(10):1387-1395. doi: 10.1016/j.jsps.2022.06.008. Epub 2022 Jun 21.
Muscular atrophy (MA) is a disease of various origins, i.e., genetic or the most common, caused by mechanical injury. So far, there is no universal therapeutic model because this disease is often progressive with numerous manifested symptoms. Moreover, there is no safe and low-risk therapy dedicated to muscle atrophy. For this reason, our research focuses on finding an alternative method using natural compounds to treat MA. This study proposes implementing natural substances such as celastrol and Rhynchophylline on the cellular level, using a simulated and controlled atrophy process. Celastrol and Rhynchophylline were used as natural compounds against simulated atrophy in C2C12 cells. Skeletal muscle C2C12 cells were stimulated for the differentiation process. Atrophic conditions were obtained by the exposure to the low concertation of doxorubicin and validated by FoxO3 and MAFbx. The protective and regenerative effect of drugs on cell proliferation was determined by the MTT assay and MT-CO1, VDAC1, and prohibitin expression. The obtained results revealed that both natural substances reduced atrophic symptoms. Rhynchophylline and celastrol attenuated atrophic cells in the viability studies, morphology analysis by diameter measurements, modulated prohibitin VDAC, and MT-CO1 expression. The obtained results revealed that celastrol and Rhynchophylline could be effectively used as a supportive treatment in atrophy-related disorders. Thus, natural drugs seem promising for muscle regeneration.
肌肉萎缩（MA）是一种由多种原因引起的疾病，即遗传因素或最常见的机械损伤所致。到目前为止，还没有通用的治疗模式，因为这种疾病通常呈进行性发展，有许多明显的症状。此外，还没有专门针对肌肉萎缩的安全且低风险的治疗方法。因此，我们的研究重点是寻找一种使用天然化合物治疗MA的替代方法。本研究建议在细胞水平上使用天然物质如雷公藤红素和钩藤碱，采用模拟和可控的萎缩过程。雷公藤红素和钩藤碱被用作针对C2C12细胞模拟萎缩的天然化合物。刺激骨骼肌C2C12细胞进行分化过程。通过暴露于低浓度阿霉素获得萎缩条件，并通过FoxO3和MAFbx进行验证。通过MTT法以及MT-CO1、VDAC1和抑制素表达来确定药物对细胞增殖的保护和再生作用。所得结果表明，这两种天然物质都减轻了萎缩症状。在活力研究、通过直径测量进行的形态分析、调节抑制素VDAC和MT-CO1表达方面，钩藤碱和雷公藤红素减轻了萎缩细胞。所得结果表明雷公藤红素和钩藤碱可有效用作萎缩相关疾病的辅助治疗。因此，天然药物在肌肉再生方面似乎很有前景。
