Matic Svjetlana, Dia Vermont P
Department of Food Science, The University of Tennessee, Institute of Agriculture, Knoxville, TN, 37996, USA.
Curr Res Food Sci. 2022 Nov 5;5:2108-2113. doi: 10.1016/j.crfs.2022.11.002. eCollection 2022.
Exosomes are extracellular vesicles implicated in cell-to-cell communication. The objective was to investigate the effect of exosomes in macrophages under hypoxia. Exosomes were isolated from skim milk using differential centrifugation and was characterized by particle size and exosomal markers TSG101, CD81, and ALIX. The effect of exosomes on macrophages under hypoxia was investigated by assessing proliferation, cytokine and reactive oxygen species (ROS) production, and cell cycle. Exosomes treatment increased the cell viability under hypoxia while ROS production was significantly reduced. The production of TNF-α was not affected by hypoxia alone but increased in a dose-dependent manner in cells treated with exosomes under hypoxic condition. Hypoxia arrested cells in the G0/G1 phase whereas exosome treatment reduced the cell in this phase. Our study found that bovine milk exosomes affect the proliferation of macrophages under hypoxia and are able to reverse the adverse effects of hypoxia on cell viability.
外泌体是参与细胞间通讯的细胞外囊泡。目的是研究外泌体在缺氧条件下对巨噬细胞的影响。使用差速离心法从脱脂乳中分离出外泌体，并通过粒径和外泌体标志物TSG101、CD81和ALIX对其进行表征。通过评估增殖、细胞因子和活性氧（ROS）产生以及细胞周期，研究了外泌体在缺氧条件下对巨噬细胞的影响。外泌体处理增加了缺氧条件下的细胞活力，同时ROS产生显著减少。TNF-α的产生不受单独缺氧的影响，但在缺氧条件下用外泌体处理的细胞中以剂量依赖性方式增加。缺氧使细胞停滞在G0/G1期，而外泌体处理减少了处于该期的细胞。我们的研究发现，牛乳外泌体影响缺氧条件下巨噬细胞的增殖，并能够逆转缺氧对细胞活力的不利影响。
