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CD73的高表达通过促进细胞增殖和迁移导致透明细胞肾细胞癌预后不良。

High expression of CD73 contributes to poor prognosis of clear-cell renal cell carcinoma by promoting cell proliferation and migration.

作者信息

Zhou Yihong, Jiang Dong, Chu Xi, Yan Minbo, Qi Hao, Wu Xiang, Tang Yuxin, Dai Yingbo

机构信息

Department of Urology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China.

Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, China.

出版信息

Transl Cancer Res. 2022 Oct;11(10):3634-3644. doi: 10.21037/tcr-22-544.

DOI:10.21037/tcr-22-544
PMID:36388013
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9641103/
Abstract

BACKGROUND

Accumulating data have shown that high expression of CD73 is associated with poor prognosis in various cancers, however the role and significance of CD73 in clear-cell renal cell carcinoma (ccRCC) still remain unclear. The present study aims to evaluate the prognostic significance of CD73 in ccRCC and explore the potential function and .

METHODS

Firstly, the expression of CD73 in ccRCC was detected using clinical tissues and verified using TCGA and GEO data. Immunohistochemistry and Kaplan-Meier test were performed for survival analysis. Furthermore, knockdown or overexpression of CD73 was conducted by lentivirus transfection in ccRCC cells. MTT assay, colony formation assay, wound healing assay, transwell assay and xenograft assay were performed or .

RESULTS

Our results showed that CD73 was highly expressed in ccRCC, and high expression of CD73 was negatively correlated with prognosis. In addition, CD73 promoted cell proliferation and migration and . Our data also showed that CD73 played both enzymatic and non-enzymatic functions in the regulation of cell proliferation and migration in ccRCC.

CONCLUSIONS

These findings suggested that CD73 might promote the growth of ccRCC and contribute to poor prognosis. Taken together, CD73 may be a potential therapeutic target in ccRCC.

摘要

背景

越来越多的数据表明，CD73的高表达与多种癌症的不良预后相关，然而CD73在透明细胞肾细胞癌（ccRCC）中的作用和意义仍不清楚。本研究旨在评估CD73在ccRCC中的预后意义，并探索其潜在功能。

方法

首先，利用临床组织检测ccRCC中CD73的表达，并使用TCGA和GEO数据进行验证。进行免疫组织化学和Kaplan-Meier试验以进行生存分析。此外，通过慢病毒转染在ccRCC细胞中进行CD73的敲低或过表达。进行MTT试验、集落形成试验、伤口愈合试验、Transwell试验和异种移植试验。

结果

我们的结果表明，CD73在ccRCC中高表达，且CD73的高表达与预后呈负相关。此外，CD73促进细胞增殖和迁移。我们的数据还表明，CD73在ccRCC细胞增殖和迁移的调节中发挥酶促和非酶促功能。

结论

这些发现表明，CD73可能促进ccRCC的生长并导致不良预后。综上所述，CD73可能是ccRCC的一个潜在治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67d8/9641103/5953e9bd1ca1/tcr-11-10-3634-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67d8/9641103/e50a3182425b/tcr-11-10-3634-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67d8/9641103/f9cee3c08765/tcr-11-10-3634-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67d8/9641103/1abec419d804/tcr-11-10-3634-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67d8/9641103/f2a4348d9f30/tcr-11-10-3634-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67d8/9641103/5953e9bd1ca1/tcr-11-10-3634-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67d8/9641103/e50a3182425b/tcr-11-10-3634-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67d8/9641103/f9cee3c08765/tcr-11-10-3634-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67d8/9641103/1abec419d804/tcr-11-10-3634-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67d8/9641103/f2a4348d9f30/tcr-11-10-3634-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67d8/9641103/5953e9bd1ca1/tcr-11-10-3634-f5.jpg

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