Fattorini Neri, Maier Uwe G
Department of Biology, Laboratory for Cell Biology Philipps-University Marburg, Marburg, Germany.
Center for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, Marburg, Germany.
Front Plant Sci. 2022 Oct 28;13:1006072. doi: 10.3389/fpls.2022.1006072. eCollection 2022.
The frustule of diatoms has an exceptional structure composed of inorganic and organic molecules. In the organic fraction, protein families were identified whose members are expected to have a complex cellular targeting to their final location within the frustule. Here we investigated for frustule-targeting signals two representatives of the cingulin family, the proteins CinY2 and CinW2; beside an already known, classical signal peptide, we have identified further regions involved in cellular targeting. By using these regions as a search criteria we were able to identify two new frustule proteins. In addition, we showed that the temporal regulation of the gene expression determines the final location of one cingulin. Our results therefore point to a sophisticated cellular and extracellular targeting of frustule components to build the fascinating frustule structure of a diatom.
硅藻的壳面具有由无机和有机分子组成的特殊结构。在有机部分中，已鉴定出蛋白质家族，其成员预计在壳面内具有复杂的细胞靶向定位至其最终位置。在这里，我们研究了扣带蛋白家族的两个代表蛋白CinY2和CinW2的壳面靶向信号；除了一个已知的经典信号肽外，我们还鉴定出了参与细胞靶向定位的其他区域。通过将这些区域用作搜索标准，我们能够鉴定出两种新的壳面蛋白。此外，我们表明基因表达的时间调控决定了一种扣带蛋白的最终位置。因此，我们的结果表明，硅藻的壳面成分通过复杂的细胞内和细胞外靶向定位来构建迷人的硅藻壳面结构。
