LeDuff Paul, Roesijadi Guritno, Rorrer Gregory L
School of Chemical, Biological, and Environmental Engineering, Oregon State University, Corvallis, OR, 97331, USA.
Department of Microbiology, Oregon State University, Corvallis, OR, 97331, USA.
Luminescence. 2016 Nov;31(7):1379-1383. doi: 10.1002/bio.3118. Epub 2016 Feb 25.
Diatoms are single-celled microalgae that possess a nanostructured, porous biosilica shell called a frustule. This study characterized the micro-photoluminescence (μ-PL) emission of single living cells of the photosynthetic marine diatom Thalassiosira pseudonana in response to UV laser irradiation at 325 nm using a confocal Raman microscope. The photoluminescence (PL) spectrum had two primary peaks, one centered at 500-510 nm, which was attributed to the frustule biosilica, and a second peak at 680 nm, which was attributed to auto-fluorescence of photosynthetic pigments. The portion of the μ-PL emission spectrum associated with biosilica frustule in the single living diatom cell was similar to that from single biosilica frustules isolated from these diatom cells. The PL emission by the biosilica frustule in the living cell emerged only after cells were cultivated to silicon depletion. The discovery of the discovery of PL emission by the frustule biosilica within a single living diatom itself, not just its isolated frustule, opens up future possibilities for living biosensor applications, where the interaction of diatom cells with other molecules can be probed by μ-PL spectroscopy. Copyright © 2016 John Wiley & Sons, Ltd.
硅藻是单细胞微藻，具有一种称为硅藻壳的纳米结构多孔生物硅外壳。本研究使用共聚焦拉曼显微镜，对光合海洋硅藻假微型海链藻的单个活细胞在325 nm紫外激光照射下的微光致发光（μ-PL）发射进行了表征。光致发光（PL）光谱有两个主要峰值，一个位于500 - 510 nm，归因于硅藻壳生物硅，另一个位于680 nm的峰值归因于光合色素的自发荧光。单个活硅藻细胞中与生物硅硅藻壳相关的μ-PL发射光谱部分与从这些硅藻细胞中分离出的单个生物硅硅藻壳的发射光谱相似。活细胞中生物硅硅藻壳的PL发射仅在细胞培养至硅耗尽后才出现。在单个活硅藻自身内部，而不仅仅是其分离出的硅藻壳中发现生物硅硅藻壳的PL发射，为活生物传感器应用开辟了未来的可能性，在这种应用中，硅藻细胞与其他分子的相互作用可以通过μ-PL光谱进行探测。版权所有© 2016约翰威立父子有限公司。
