Ciriello Michele, Formisano Luigi, Kyriacou Marios, Soteriou Georgios A, Graziani Giulia, De Pascale Stefania, Rouphael Youssef
Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy.
Department of Vegetable Crops, Agricultural Research Institute, Nicosia, Cyprus.
Front Plant Sci. 2022 Oct 27;13:1049004. doi: 10.3389/fpls.2022.1049004. eCollection 2022.
L. is an aromatic plant rich in bioactive metabolites beneficial to human health. The agronomic biofortification of basil with Zn could provide a practical and sustainable solution to address Zn deficiency in humans. Our research appraised the effects of biofortification implemented through nutrient solutions of different Zn concentration (12.5, 25.0, 37.5, and 50 µM) on the yield, physiological indices (net CO assimilation rate, transpiration, stomatal conductance, and chlorophyll fluorescence), quality, and Zn concentration of basil cultivars 'Aroma 2' and 'Eleonora' grown in a floating raft system. The ABTS, DPPH, and FRAP antioxidant activities were determined by UV-VIS spectrophotometry, the concentrations of phenolic acids by mass spectrometry using a Q Extractive Orbitrap LC-MS/MS, and tissue Zn concentration by inductively coupled plasma mass spectrometry. Although increasing the concentration of Zn in the nutrient solution significantly reduced the yield, this reduction was less evident in 'Aroma 2'. However, regardless of cultivar, the use of the maximum dose of Zn (50 µM) increased the concentration of carotenoids, polyphenols, and antioxidant activity on average by 19.76, 14.57, and 33.72%, respectively, compared to the Control. The significant positive correlation between Zn in the nutrient solution and Zn in plant tissues underscores the suitability of basil for soilless biofortification programs.
罗勒是一种富含对人体健康有益的生物活性代谢物的芳香植物。用锌对罗勒进行农艺生物强化可为解决人类锌缺乏问题提供切实可行且可持续的解决方案。我们的研究评估了通过不同锌浓度（12.5、25.0、37.5和50 μM）的营养液进行生物强化对在漂浮筏系统中种植的罗勒品种“Aroma 2”和“Eleonora”的产量、生理指标（净二氧化碳同化率、蒸腾作用、气孔导度和叶绿素荧光）、品质和锌浓度的影响。通过紫外可见分光光度法测定ABTS、DPPH和FRAP抗氧化活性，使用Q Extractive Orbitrap LC-MS/MS通过质谱法测定酚酸浓度，通过电感耦合等离子体质谱法测定组织锌浓度。尽管增加营养液中锌的浓度显著降低了产量，但这种降低在“Aroma 2”中不太明显。然而，无论品种如何，与对照相比，使用最大剂量的锌（50 μM）平均使类胡萝卜素、多酚浓度和抗氧化活性分别提高了19.76%、14.57%和33.72%。营养液中的锌与植物组织中的锌之间的显著正相关突出了罗勒适用于无土生物强化计划。
