Laboratoire Productivité Végétale et Contraintes Environnementales, Département des Sciences Biologiques, Faculté des Sciences de Tunis, Université Tunis El Manar, 2092, Tunis, Tunisia.
Laboratory of Extremophile Plants, Centre of Biotechnology of Borj-Cedria, P. O. Box 901, 2050, Hammam-Lif, Tunisia.
Bull Environ Contam Toxicol. 2021 May;106(5):899-907. doi: 10.1007/s00128-021-03188-6. Epub 2021 Apr 3.
In the present study, the effects of elevated zinc concentrations on germination, physiological and biochemical parameters were investigated in basil (Ocimum basilicum L.). Results indicate that zinc excess (1-5 mM ZnSO) did not affect germination process, but it drastically reduced vigor index and radicle elongation, and induced oxidative stress. Exposure of basil plants to 400 and 800 µM Zn decreased aerial parts and roots dry biomass, root length and leaf number. Under these conditions, the reduction of plant growth was associated with the formation of branched and abnormally shaped brown roots. Translocation factor < 1 and bioconcentration factor > 1 was observed for 100 µM Zn suggested the possible use of basil as a phytostabiliser. Excess of Zn supply (> 100 µM) decreased chlorophyll content, total phenol and total flavonoid contents. Additionally, an increased TBARS levels reflecting an oxidative burst was observed in Zn-treated plants. These findings suggest that excess Zn adversely affects plant growth, photosynthetic pigments, phenolic and flavonoid contents, and enhances oxidative stress in basil plants.
在本研究中，研究了锌浓度升高对罗勒（Ocimum basilicum L.）种子萌发、生理和生化参数的影响。结果表明，锌过量（1-5 mM ZnSO）并不影响萌发过程，但严重降低了活力指数和胚根伸长率，并诱导了氧化应激。暴露于 400 和 800 μM Zn 会降低植株地上部分和根系干重、根长和叶片数。在这些条件下，植物生长的减少与形成分枝和形状异常的棕色根有关。100 μM Zn 的转运因子<1 和生物浓缩因子>1 表明罗勒可能被用作植物稳定器。过量的锌供应（>100 μM）会降低叶绿素、总酚和总类黄酮的含量。此外，在锌处理的植物中观察到 TBARS 水平升高，反映出氧化爆发。这些发现表明，过量的锌会对罗勒植物的生长、光合色素、酚类和类黄酮含量产生不利影响，并增强氧化应激。
