Nacoon Sabaiporn, Seemakram Wasan, Ekprasert Jindarat, Jogloy Sanun, Kuyper Thomas W, Mongkolthanaruk Wiyada, Riddech Nuntavun, Somdee Theerasak, Boonlue Sophon
Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.
Department of Agronomy, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
Front Plant Sci. 2022 Oct 31;13:1022319. doi: 10.3389/fpls.2022.1022319. eCollection 2022.
Due to different functions of phosphate solubilizing bacteria (PSB) and arbuscular mycorrhizal fungi (AMF), their potential synergistic effects on enhancing plant growth and yield are worth investigating, especially under adverse conditions. This work focused on the isolation of PSB and characterization for their plant growth promoting properties under drought. The most efficient P solubilizing bacterium was isolated and identified as strain KKUT8-1. Then, a factorial experiment on the performance of sunchoke () was set up with four factors, viz., PSB (presence or absence of KKUT8-1), AMF (presence or absence of ), rock phosphate (RP; added or not) and moisture (well-watered (WW) or drought (DS) conditions). Sunchoke performance was enhanced by the presence of AMF, whereas addition of PSB had a positive effect on SPAD values and inulin concentration. Drought reduced plant performance, while addition of RP reduced photosynthetic rate. There was little evidence for synergistic effects between PSB and AMF, except for SPAD values and inulin concentration. Plants that were co-inoculated with AMF and PSB had highest SPAD value, shoot diameter, leaf area, leaf number, chlorophyll concentration, plant biomass, tuber production, root growth and total soluble sugar concentration. Co-inoculated plants also had increased plant water status, reduced electrolyte leakage, and reduced malondialdehyde and proline concentration. Strain KKUT8-1 is the first strain of capable of promoting growth and yield of sunchoke. Enhanced production of sunchoke by a combination of AMF and PSB was much better than the application of RP. Our finding offers an opportunity to develop combinations of biological inoculants for increasing the growth and production of sunchoke under drought in the future.
由于解磷细菌（PSB）和丛枝菌根真菌（AMF）的功能不同，它们在促进植物生长和提高产量方面的潜在协同效应值得研究，尤其是在不利条件下。这项工作的重点是解磷细菌的分离及其在干旱条件下促进植物生长特性的表征。分离出了最有效的解磷细菌并鉴定为KKUT8-1菌株。然后，以菊芋（）为研究对象，设置了一个析因实验，包含四个因素，即PSB（有无KKUT8-1）、AMF（有无）、磷矿粉（RP；添加与否）和水分（充分浇水（WW）或干旱（DS）条件）。AMF的存在提高了菊芋的生长性能，而添加PSB对SPAD值和菊粉浓度有积极影响。干旱降低了植物的生长性能，而添加RP降低了光合速率。除了SPAD值和菊粉浓度外，几乎没有证据表明PSB和AMF之间存在协同效应。同时接种AMF和PSB的植物具有最高的SPAD值、茎直径、叶面积、叶片数量、叶绿素浓度、植物生物量、块茎产量、根系生长和总可溶性糖浓度。同时接种的植物还具有更高的植物水分状况、更低的电解质渗漏率，以及更低的丙二醛和脯氨酸浓度。KKUT8-1菌株是首个能够促进菊芋生长和提高产量的菌株。AMF和PSB组合提高菊芋产量的效果远优于磷矿粉的施用。我们 的研究结果为未来开发生物接种剂组合以提高干旱条件下菊芋的生长和产量提供了契机。
