非带电转运RNA错误抑制模型。

Uncharged tRNA error damping model.

作者信息

Gallant J A

出版信息

FEBS Lett. 1986 Oct 6;206(2):185-8. doi: 10.1016/0014-5793(86)80977-2.

Abstract

Uncharged tRNA is known to bind to the ribosome in a codon-specific fashion. In this way, cognate uncharged tRNA competes with non-cognate aminoacyl-tRNA. If uncharged tRNA can be aminoacylated while on the ribosome, this will damp errors due to aminoacyl-tRNA imbalance. Kinetic analysis shows that this scheme reduces errors at 'hungry' codons considerably more effectively than J. Ninio's accuracy tuner model; for example, a 10-fold decrease in cognate aminoacyl-tRNA elicits only a 10% increase in the error frequency.

摘要

已知不带电荷的tRNA以密码子特异性方式与核糖体结合。通过这种方式，同源的不带电荷的tRNA与非同源的氨酰tRNA竞争。如果不带电荷的tRNA在核糖体上时能够被氨酰化，这将减少由于氨酰tRNA失衡导致的错误。动力学分析表明，该方案在“饥饿”密码子处减少错误的效果比J. Ninio的准确性调节模型更有效得多；例如，同源氨酰tRNA减少10倍只会导致错误频率增加10%。