Cauduro Guilherme Pinto, Marmitt Marcela, Ferraz Marlon, Arend Sabrina Nicole, Kern Gabriela, Modolo Regina Célia Espinosa, Leal Ana Lusia, Valiati Victor Hugo
Laboratory of Genetics and Molecular Biology, Programa de Pós-Graduação Em Biologia, Universidade Do Vale Do Rio Dos Sinos (UNISINOS), Av. Unisinos 950, São Leopoldo, RS, 93022-750, Brazil.
Laboratory of Fish Ecology, Programa de Pós-Graduação Em Biologia, Universidade Do Vale Do Rio Dos Sinos (UNISINOS), São Leopoldo, RS, Brazil.
Environ Monit Assess. 2022 Nov 17;195(1):116. doi: 10.1007/s10661-022-10733-1.
Polycyclic aromatic hydrocarbons (PAHs) are one of the main pollutants generated by the refining and use of oil. To search bioremediation alternatives for these compounds, mainly in situ, considering the biotic and abiotic variables that affect the contaminated sites is determinant for the success of bioremediation techniques. In this study, bioremediation strategies were evaluated in situ, including biostimulation and bioaugmentation for 16 priority PAHs present in activated sludge farms. B. vietnamiensis G4 was used as a biodegradation agent for bioaugmentation tests. The analyses occurred for 12 months, and temperature and humidity were measured to verify the effects of these factors on the biodegradation. We used the technique GC-MS to evaluate and quantify the degradation of PAHs over the time of the experiment. Of the four treatments applied, bioaugmentation with quarterly application proved to be the best strategy, showing the degradation of compounds of high (34.4% annual average) and low (21.9% annual average) molecular weight. A high degradation rate for high molecular weight compounds demonstrates that this technique can be successfully applied in bioremediation of areas with compounds considered toxic and stable in nature, contributing to the mitigation of impacts generated by PAHs.
多环芳烃（PAHs）是石油提炼和使用过程中产生的主要污染物之一。为了寻找这些化合物的生物修复替代方法，主要是原位修复，考虑影响受污染场地的生物和非生物变量对于生物修复技术的成功至关重要。在本研究中，对活性污泥场中存在的16种优先PAHs进行了原位生物修复策略评估，包括生物刺激和生物强化。越南伯克霍尔德氏菌G4被用作生物强化试验的生物降解剂。分析进行了12个月，并测量了温度和湿度，以验证这些因素对生物降解的影响。我们使用气相色谱-质谱联用（GC-MS）技术来评估和量化实验期间PAHs的降解情况。在所应用的四种处理方法中，每季度进行一次生物强化被证明是最佳策略，显示出高分子量（年平均降解率34.4%）和低分子量（年平均降解率21.9%）化合物的降解。高分子量化合物的高降解率表明，该技术可成功应用于对具有毒性且性质稳定的化合物区域的生物修复，有助于减轻PAHs产生的影响。
