Otieno S, Bloor A, Karol R, Reichlin M, Noble R W
J Immunol. 1978 Dec;121(6):2458-62.
Like goats and sheep, guinea pigs can produce, in response to human sickle cell hemoglobin (beta6 Glu leads to Val), an antibody population (anti-Val) that will bind sickle cell hemoglobin but not normal hemoglobin HbA. Unlike goats and sheep, guinea pigs can produce in response to human hemoglobin A1 an antibody fraction, anti-Glu, that will not react with human sickle cell hemoglobin. These anti-Glu antibodies have been isolated by affinity chromatography and their specificity confirmed by fluorescence-quenching titrations. The sequence of the first 10 amino acids of the beta-chain of guinea pig hemoglobin has been determined. This sequence differs from those of both hemoglobin HbA and sickle cell hemoglobin by two residues, those at positions 5 and 6. This explains the similarity of the immunogenicity of this site on the two human hemoglobins when administered to guinea pigs. Both goats and sheep are identical to hemoglobin A1 at the beta-6 position.
与山羊和绵羊一样，豚鼠能够针对人类镰状细胞血红蛋白（β6 谷氨酸突变为缬氨酸）产生一群抗体（抗缬氨酸抗体），该抗体能结合镰状细胞血红蛋白，但不结合正常血红蛋白 HbA。与山羊和绵羊不同的是，豚鼠能够针对人类血红蛋白 A1 产生一种抗体组分，即抗谷氨酸抗体，该抗体不会与人类镰状细胞血红蛋白发生反应。这些抗谷氨酸抗体已通过亲和层析法分离出来，其特异性通过荧光猝灭滴定法得以证实。豚鼠血红蛋白β链前 10 个氨基酸的序列已被确定。该序列与血红蛋白 HbA 和镰状细胞血红蛋白的序列在两个残基上存在差异，即第 5 和第 6 位的残基。这就解释了将这两个人类血红蛋白的该位点给予豚鼠时免疫原性相似的原因。山羊和绵羊在β-6 位置与血红蛋白 A1 相同。
